Identification of a vesicular nucleotide transporter

Keisuke Sawada, Noriko Echigo, Narinobu Juge, Takaaki Miyaji, Masato Otsuka, Hiroshi Omote, Akitsugu Yamamoto, Yoshinori Moriyama

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

ATP is a major chemical transmitter in purinergic signal transmission. Before secretion, ATP is stored in secretory vesicles found in purinergic cells. Although the presence of active transport mechanisms for ATP has been postulated for a long time, the proteins responsible for its vesicular accumulation remains unknown. The transporter encoded by the human and mouse SLC17A9 gene, a novel member of an anion transporter family, was predominantly expressed in the brain and adrenal gland. The mouse and bovine counterparts were associated with adrenal chromaffin granules. Proteoliposomes containing purified transporter actively took up ATP, ADP, and GTP by using membrane potential as the driving force. The uptake properties of the reconstituted transporter were similar to that of the ATP uptake by synaptic vesicles and chromaffin granules. Suppression of endogenous SLC17A9 expression in PC12 cells decreased exocytosis of ATP. These findings strongly suggest that SLC17A9 protein is a vesicular nucleotide transporter and should lead to the elucidation of the molecular mechanism of ATP secretion in purinergic signal transmission.

Original languageEnglish
Pages (from-to)5683-5686
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number15
DOIs
Publication statusPublished - Apr 15 2008

Fingerprint

Nucleotides
Adenosine Triphosphate
Chromaffin Granules
Synaptic Vesicles
Active Biological Transport
PC12 Cells
Exocytosis
Secretory Vesicles
Adrenal Glands
Guanosine Triphosphate
Membrane Potentials
Adenosine Diphosphate
Anions
Proteins
Brain
Genes

Keywords

  • ATP
  • Chromaffin granule
  • Purinergic signaling
  • Storage and exocytosis
  • Synaptic vesicle

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of a vesicular nucleotide transporter. / Sawada, Keisuke; Echigo, Noriko; Juge, Narinobu; Miyaji, Takaaki; Otsuka, Masato; Omote, Hiroshi; Yamamoto, Akitsugu; Moriyama, Yoshinori.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 15, 15.04.2008, p. 5683-5686.

Research output: Contribution to journalArticle

Sawada, Keisuke ; Echigo, Noriko ; Juge, Narinobu ; Miyaji, Takaaki ; Otsuka, Masato ; Omote, Hiroshi ; Yamamoto, Akitsugu ; Moriyama, Yoshinori. / Identification of a vesicular nucleotide transporter. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 15. pp. 5683-5686.
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AU - Miyaji, Takaaki

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AU - Omote, Hiroshi

AU - Yamamoto, Akitsugu

AU - Moriyama, Yoshinori

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