Identification of a vesicular aspartate transporter

Takaaki Miyaji, Noriko Echigo, Miki Hiasa, Shigenori Senoh, Hiroshi Omote, Yoshinori Moriyama

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Aspartate is an excitatory amino acid that is costored with glutamate in synaptic vesicles of hippocampal neurons and synaptic-like microvesicles (SLMVs) of pinealocytes and is exocytosed and stimulates neighboring cells by binding to specific cell receptors. Although evidence increasingly supports the occurrence of aspartergic neurotransmission, this process is still debated because the mechanism for the vesicular storage of aspartate is unknown. Here, we show that sialin, a lysosomal H+/sialic acid cotransporter, is present in hippocampal synaptic vesicles and pineal SLMVs. RNA interference of sialin expression decreased exocytosis of aspartate and glutamate in pinealocytes. Proteoliposomes containing purified sialin actively accumulated aspartate and glutamate to a similar extent when inside positive membrane potential is imposed as the driving force. Sialin carrying a mutation found in people suffering from Salla disease (R39C) was completely devoid of aspartate and glutamate transport activity, although it retained appreciable H +/sialic acid cotransport activity. These results strongly suggest that sialin possesses dual physiological functions and acts as a vesicular aspartate/glutamate transporter. It is possible that people with Salla disease lose aspartergic (and also the associated glutamatergic) neurotransmission, and this could provide an explanation for why Salla disease causes severe neurological defects.

Original languageEnglish
Pages (from-to)11720-11724
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number33
DOIs
Publication statusPublished - Aug 19 2008

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Aspartic Acid
Sialic Acid Storage Disease
Glutamic Acid
Synaptic Vesicles
N-Acetylneuraminic Acid
Synaptic Transmission
Vesicular Glutamate Transport Proteins
Excitatory Amino Acids
Exocytosis
RNA Interference
Membrane Potentials
Neurons
Mutation

Keywords

  • Salla disease
  • Sialin
  • Vesicular excitatory amino acid transporter
  • Vesicular glutamate transporter

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of a vesicular aspartate transporter. / Miyaji, Takaaki; Echigo, Noriko; Hiasa, Miki; Senoh, Shigenori; Omote, Hiroshi; Moriyama, Yoshinori.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 33, 19.08.2008, p. 11720-11724.

Research output: Contribution to journalArticle

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