Identification of a unique antigen peptide pRL1 on BALB/c RL♂1 leukemia recognized by cytotoxic T lymphocytes and its relation to the Akt oncogene

Akiko Uenaka, Toshiro Ono, Toshifumi Akisawa, Hisashi Wada, Tatsuji Yasuda, Eiichi Nakayama

Research output: Contribution to journalArticle

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Abstract

BALB/c radiation leukemia RL♂1 is an immunogenic tumor. We established bulk and cloned cytotoxic T lymphocyte (CTL) lines from regressor (BALB/c x C57BL/6)F1 (CB6F1) spleen cells that recognized RL♂1 specifically. We then obtained antigen peptide recognized by CTL from RL♂1 by acid extraction. Analysis of the acid extract by reversed-phase high performance liquid chromatography (HPLC) on a semipreparative C18 column revealed that fractions eluted in 23 min (peak a) and 26 min (peak b) showed sensitization activity on the P815 target for specific CTL. On further purification of these fractions by HPLC and direct sequencing by Edman degradation, we identified the CTL-recognizing RL♂1 peptide pRL1a (IPGLPLSL) in peak a and its possible precursor peptide pRL1b (SIIPGLPLSL) in peak b. Sequence homology indicated that these peptides were derived from the 5' untranslated region of c-akt oncogene.

Original languageEnglish
Pages (from-to)1599-1607
Number of pages9
JournalJournal of Experimental Medicine
Volume180
Issue number5
DOIs
Publication statusPublished - Nov 1 1994

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Cytotoxic T-Lymphocytes
Oncogenes
Leukemia
Antigens
Peptides
High Pressure Liquid Chromatography
Acids
5' Untranslated Regions
Reverse-Phase Chromatography
Sequence Homology
Spleen
Radiation
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of a unique antigen peptide pRL1 on BALB/c RL♂1 leukemia recognized by cytotoxic T lymphocytes and its relation to the Akt oncogene. / Uenaka, Akiko; Ono, Toshiro; Akisawa, Toshifumi; Wada, Hisashi; Yasuda, Tatsuji; Nakayama, Eiichi.

In: Journal of Experimental Medicine, Vol. 180, No. 5, 01.11.1994, p. 1599-1607.

Research output: Contribution to journalArticle

Uenaka, Akiko ; Ono, Toshiro ; Akisawa, Toshifumi ; Wada, Hisashi ; Yasuda, Tatsuji ; Nakayama, Eiichi. / Identification of a unique antigen peptide pRL1 on BALB/c RL♂1 leukemia recognized by cytotoxic T lymphocytes and its relation to the Akt oncogene. In: Journal of Experimental Medicine. 1994 ; Vol. 180, No. 5. pp. 1599-1607.
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