Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads

Hideaki Takeuchi, A. Yasuda, Y. Yasuda-Kamatani, T. Kubo, Terumi Nakajima

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Using a combination of MALDI-TOF and on-line capillary HPLC/Q-Tof mass spectroscopy, we identified and determined the amino acid sequence of a novel neuropeptide in the brain of the honeybee Apis mellifera L., termed AmTRP peptide (Apis mellifera tachykinin-related peptide), related to insect tachykinin. A cDNA for a prepro-protein (prepro-AmTRP) of AmTRP was isolated and determined to encode seven AmTRPs 1-7. Northern blot analysis indicated that the prepro-AmTRP gene is expressed differentially in the nurse bee, forager, queen and drone heads. Strong expression was detected in the queen and forager heads, while weak and almost no significant expression was detected in the nurse and drone heads, respectively. These results suggest that AmTRP peptide functions as a neuromodulator and/or hormone, associated with sex-specific or age/division of labour-selective behaviour and/or physiology of the honeybees.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalInsect Molecular Biology
Volume12
Issue number3
DOIs
Publication statusPublished - Jun 2003
Externally publishedYes

Fingerprint

Tachykinins
drones (insects)
matrix-assisted laser desorption-ionization mass spectrometry
Bees
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
neuropeptides
Neuropeptides
queen insects
Gene expression
honey bees
Brain
Head
peptides
nurses
brain
Gene Expression
Apis mellifera
gene expression
Peptides
Supervisory Nursing

Keywords

  • Behaviour
  • Gene expression
  • Honeybee
  • MALDI-TOF MS
  • Mushroom body
  • Tachykinin

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads. / Takeuchi, Hideaki; Yasuda, A.; Yasuda-Kamatani, Y.; Kubo, T.; Nakajima, Terumi.

In: Insect Molecular Biology, Vol. 12, No. 3, 06.2003, p. 291-298.

Research output: Contribution to journalArticle

@article{674e177378b5459a96b628ba05fac97b,
title = "Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads",
abstract = "Using a combination of MALDI-TOF and on-line capillary HPLC/Q-Tof mass spectroscopy, we identified and determined the amino acid sequence of a novel neuropeptide in the brain of the honeybee Apis mellifera L., termed AmTRP peptide (Apis mellifera tachykinin-related peptide), related to insect tachykinin. A cDNA for a prepro-protein (prepro-AmTRP) of AmTRP was isolated and determined to encode seven AmTRPs 1-7. Northern blot analysis indicated that the prepro-AmTRP gene is expressed differentially in the nurse bee, forager, queen and drone heads. Strong expression was detected in the queen and forager heads, while weak and almost no significant expression was detected in the nurse and drone heads, respectively. These results suggest that AmTRP peptide functions as a neuromodulator and/or hormone, associated with sex-specific or age/division of labour-selective behaviour and/or physiology of the honeybees.",
keywords = "Behaviour, Gene expression, Honeybee, MALDI-TOF MS, Mushroom body, Tachykinin",
author = "Hideaki Takeuchi and A. Yasuda and Y. Yasuda-Kamatani and T. Kubo and Terumi Nakajima",
year = "2003",
month = "6",
doi = "10.1046/j.1365-2583.2003.00414.x",
language = "English",
volume = "12",
pages = "291--298",
journal = "Insect Molecular Biology",
issn = "0962-1075",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads

AU - Takeuchi, Hideaki

AU - Yasuda, A.

AU - Yasuda-Kamatani, Y.

AU - Kubo, T.

AU - Nakajima, Terumi

PY - 2003/6

Y1 - 2003/6

N2 - Using a combination of MALDI-TOF and on-line capillary HPLC/Q-Tof mass spectroscopy, we identified and determined the amino acid sequence of a novel neuropeptide in the brain of the honeybee Apis mellifera L., termed AmTRP peptide (Apis mellifera tachykinin-related peptide), related to insect tachykinin. A cDNA for a prepro-protein (prepro-AmTRP) of AmTRP was isolated and determined to encode seven AmTRPs 1-7. Northern blot analysis indicated that the prepro-AmTRP gene is expressed differentially in the nurse bee, forager, queen and drone heads. Strong expression was detected in the queen and forager heads, while weak and almost no significant expression was detected in the nurse and drone heads, respectively. These results suggest that AmTRP peptide functions as a neuromodulator and/or hormone, associated with sex-specific or age/division of labour-selective behaviour and/or physiology of the honeybees.

AB - Using a combination of MALDI-TOF and on-line capillary HPLC/Q-Tof mass spectroscopy, we identified and determined the amino acid sequence of a novel neuropeptide in the brain of the honeybee Apis mellifera L., termed AmTRP peptide (Apis mellifera tachykinin-related peptide), related to insect tachykinin. A cDNA for a prepro-protein (prepro-AmTRP) of AmTRP was isolated and determined to encode seven AmTRPs 1-7. Northern blot analysis indicated that the prepro-AmTRP gene is expressed differentially in the nurse bee, forager, queen and drone heads. Strong expression was detected in the queen and forager heads, while weak and almost no significant expression was detected in the nurse and drone heads, respectively. These results suggest that AmTRP peptide functions as a neuromodulator and/or hormone, associated with sex-specific or age/division of labour-selective behaviour and/or physiology of the honeybees.

KW - Behaviour

KW - Gene expression

KW - Honeybee

KW - MALDI-TOF MS

KW - Mushroom body

KW - Tachykinin

UR - http://www.scopus.com/inward/record.url?scp=0038322115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038322115&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2583.2003.00414.x

DO - 10.1046/j.1365-2583.2003.00414.x

M3 - Article

C2 - 12752663

AN - SCOPUS:0038322115

VL - 12

SP - 291

EP - 298

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 0962-1075

IS - 3

ER -