Identification of a retroelement-containing human transcript induced in the nucleus by vaccination

Tomoyuki Honda, Keiko Takemoto, Keiji Ueda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Endogenous retroelements constitute almost half of the mammalian genome. Given that more than 60% of human genomic bases are transcribed, transcripts containing these retroelements may impact various biological processes. However, the physiological roles of most retroelement-containing transcripts are yet to be revealed. Here, we profiled the expression of retroelement-containing human transcripts during vaccination and found that vaccination upregulated transcripts containing only particular retroelements, such as the MLT-int element of endogenous retroviruses. MLT-int-containing transcripts were distributed mainly in the nucleus, suggesting their unique roles in the nucleus. Furthermore, we demonstrated that MLT-int RNA suppressed interferon promoter activity in the absence of immune stimuli. Based on these lines of evidence, we speculate a model of a role of the previously unnoticedMLT-int element in preventing excess innate immune activation after elimination of immune stimuli. Our results may emphasize the importance of retroelement-containing transcripts in maintaining host immune homeostasis.

Original languageEnglish
Article number2875
JournalInternational journal of molecular sciences
Volume20
Issue number12
DOIs
Publication statusPublished - Jun 2 2019
Externally publishedYes

Keywords

  • Endogenous retrovirus
  • Innate immunity
  • Interferon
  • MLT-int
  • Retroelement
  • Vaccination

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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