Identification of a Phytase gene in barley (Hordeum vulgare L.)

Fei Dai, Long Qiu, Lingzhen Ye, Dezhi Wu, Meixue Zhou, Guoping Zhang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity. Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy. Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.

Original languageEnglish
Article numbere18829
JournalPLoS One
Volume6
Issue number4
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

6-Phytase
phytases
Hordeum
Hordeum vulgare
Genes
barley
Quantitative Trait Loci
quantitative trait loci
genes
barley products
Chromosomes, Human, Pair 5
Phytic Acid
Haploidy
Firearms
doubled haploids
Chromosomes
phytic acid
acid phosphatase
Nutrients
Breeding

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Dai, F., Qiu, L., Ye, L., Wu, D., Zhou, M., & Zhang, G. (2011). Identification of a Phytase gene in barley (Hordeum vulgare L.). PLoS One, 6(4), [e18829]. https://doi.org/10.1371/journal.pone.0018829

Identification of a Phytase gene in barley (Hordeum vulgare L.). / Dai, Fei; Qiu, Long; Ye, Lingzhen; Wu, Dezhi; Zhou, Meixue; Zhang, Guoping.

In: PLoS One, Vol. 6, No. 4, e18829, 2011.

Research output: Contribution to journalArticle

Dai, F, Qiu, L, Ye, L, Wu, D, Zhou, M & Zhang, G 2011, 'Identification of a Phytase gene in barley (Hordeum vulgare L.)', PLoS One, vol. 6, no. 4, e18829. https://doi.org/10.1371/journal.pone.0018829
Dai, Fei ; Qiu, Long ; Ye, Lingzhen ; Wu, Dezhi ; Zhou, Meixue ; Zhang, Guoping. / Identification of a Phytase gene in barley (Hordeum vulgare L.). In: PLoS One. 2011 ; Vol. 6, No. 4.
@article{d252ab4898c2490fb16290275d82147b,
title = "Identification of a Phytase gene in barley (Hordeum vulgare L.)",
abstract = "Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity. Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy. Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.",
author = "Fei Dai and Long Qiu and Lingzhen Ye and Dezhi Wu and Meixue Zhou and Guoping Zhang",
year = "2011",
doi = "10.1371/journal.pone.0018829",
language = "English",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

TY - JOUR

T1 - Identification of a Phytase gene in barley (Hordeum vulgare L.)

AU - Dai, Fei

AU - Qiu, Long

AU - Ye, Lingzhen

AU - Wu, Dezhi

AU - Zhou, Meixue

AU - Zhang, Guoping

PY - 2011

Y1 - 2011

N2 - Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity. Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy. Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.

AB - Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity. Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy. Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.

UR - http://www.scopus.com/inward/record.url?scp=79955444314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955444314&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0018829

DO - 10.1371/journal.pone.0018829

M3 - Article

C2 - 21533044

AN - SCOPUS:79955444314

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 4

M1 - e18829

ER -