Identification of a Phytase gene in barley (Hordeum vulgare L.)

Fei Dai, Long Qiu, Lingzhen Ye, Dezhi Wu, Meixue Zhou, Guoping Zhang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity. Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy. Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.

Original languageEnglish
Article numbere18829
JournalPloS one
Volume6
Issue number4
DOIs
Publication statusPublished - May 3 2011

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Dai, F., Qiu, L., Ye, L., Wu, D., Zhou, M., & Zhang, G. (2011). Identification of a Phytase gene in barley (Hordeum vulgare L.). PloS one, 6(4), [e18829]. https://doi.org/10.1371/journal.pone.0018829