Abstract
Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.
| Original language | English |
|---|---|
| Pages (from-to) | 222-228 |
| Number of pages | 7 |
| Journal | Horticulture Journal |
| Volume | 87 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
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Keywords
- Allelic variation
- Capsaicinoid
- Frameshift mutation
- Health functional ingredient
ASJC Scopus subject areas
- Plant Science
- Horticulture
Cite this
Identification of a novel mutant pAMT allele responsible for low-pungency and capsinoid production in chili pepper : Accession ‘no. 4034’ (Capsicum Chinense). / Tanaka, Yoshiyuki; Fukuta, Shiho; Koeda, Sota; Goto, Tanjuro; Yoshida, Yuichi; Yasuba, Kenichiro.
In: Horticulture Journal, Vol. 87, No. 2, 01.01.2018, p. 222-228.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a novel mutant pAMT allele responsible for low-pungency and capsinoid production in chili pepper
T2 - Accession ‘no. 4034’ (Capsicum Chinense)
AU - Tanaka, Yoshiyuki
AU - Fukuta, Shiho
AU - Koeda, Sota
AU - Goto, Tanjuro
AU - Yoshida, Yuichi
AU - Yasuba, Kenichiro
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.
AB - Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.
KW - Allelic variation
KW - Capsaicinoid
KW - Frameshift mutation
KW - Health functional ingredient
UR - http://www.scopus.com/inward/record.url?scp=85045613813&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045613813&partnerID=8YFLogxK
U2 - 10.2503/hortj.OKD-115
DO - 10.2503/hortj.OKD-115
M3 - Article
AN - SCOPUS:85045613813
VL - 87
SP - 222
EP - 228
JO - Horticulture Journal
JF - Horticulture Journal
SN - 2189-0102
IS - 2
ER -