Identification of a novel mutant pAMT allele responsible for low-pungency and capsinoid production in chili pepper: Accession ‘no. 4034’ (Capsicum Chinense)

Yoshiyuki Tanaka, Shiho Fukuta, Sota Koeda, Tanjuro Goto, Yuichi Yoshida, Kenichiro Yasuba

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8 Citations (Scopus)

Abstract

Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.

Original languageEnglish
Pages (from-to)222-228
Number of pages7
JournalHorticulture Journal
Volume87
Issue number2
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Capsicum chinense
hot peppers
transaminases
alleles
mutants
genetic complementation test
frameshift mutation
health promotion
Capsicum
breeding
exons
genes
sequence analysis
gene expression
fruits

Keywords

  • Allelic variation
  • Capsaicinoid
  • Frameshift mutation
  • Health functional ingredient

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Cite this

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title = "Identification of a novel mutant pAMT allele responsible for low-pungency and capsinoid production in chili pepper: Accession ‘no. 4034’ (Capsicum Chinense)",
abstract = "Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.",
keywords = "Allelic variation, Capsaicinoid, Frameshift mutation, Health functional ingredient",
author = "Yoshiyuki Tanaka and Shiho Fukuta and Sota Koeda and Tanjuro Goto and Yuichi Yoshida and Kenichiro Yasuba",
year = "2018",
month = "1",
day = "1",
doi = "10.2503/hortj.OKD-115",
language = "English",
volume = "87",
pages = "222--228",
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TY - JOUR

T1 - Identification of a novel mutant pAMT allele responsible for low-pungency and capsinoid production in chili pepper

T2 - Accession ‘no. 4034’ (Capsicum Chinense)

AU - Tanaka, Yoshiyuki

AU - Fukuta, Shiho

AU - Koeda, Sota

AU - Goto, Tanjuro

AU - Yoshida, Yuichi

AU - Yasuba, Kenichiro

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.

AB - Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.

KW - Allelic variation

KW - Capsaicinoid

KW - Frameshift mutation

KW - Health functional ingredient

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JO - Horticulture Journal

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