Identification of a novel adrenomedullin gene family in teleost fish

Maho Ogoshi, Koji Inoue, Yoshio Takei

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Adrenomedullin (AM) is a multifunctional peptide known to form a hormone family with calcitonin gene-related peptide (CGRP) and amylin. We have cloned five distinct AM cDNAs from the pufferfish, Takifugu rubripes, and named them TrAM-1, -2, -3, -4, and -5. Judging from the deduced precursor sequences and processing pattern of the C-terminal mature peptides, TrAMs may be divided into at least two groups; AM-2 and -3, and AM-1, -4, and possibly -5. Phylogenetic analysis of the mature peptides, exon-intron structure of their genes, and tissue distribution of their mRNA also support this classification. TrAM-1 and -4 were ubiquitously expressed in various tissues including the kidney and interrenal (adrenal homolog) as in the case of mammalian AM, while TrAM-2 and -3 were expressed most abundantly in the brain followed by the vascular tissues. Synteny of the genes around AM gene showed that TrAM-1 is the ortholog of mammalian AM. The presence of a PAMP-like sequence in the prosegment of TrAM-1 also supports this notion. Multiple AMs were also detected in another pufferfish, Tetraodon nigroviridis, and in zebrafish, Danio rerio. The present study shows for the first time the presence of a novel AM family in teleost fish that is independent from CGRP and amylin, which further suggests the possible existence of multiple AMs in mammals.

Original languageEnglish
Pages (from-to)1072-1077
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume311
Issue number4
DOIs
Publication statusPublished - Nov 28 2003
Externally publishedYes

Fingerprint

Adrenomedullin
Fish
Fishes
Genes
Tetraodontiformes
Islet Amyloid Polypeptide
Calcitonin Gene-Related Peptide
Zebrafish
Tissue
Peptides
Takifugu
Synteny
Mammals
Tissue Distribution
Introns
Blood Vessels
Exons
Brain
Complementary DNA
Hormones

Keywords

  • Adrenomedullin
  • Comparative genomics
  • Molecular evolution
  • Pufferfish
  • Takifugu rubripes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of a novel adrenomedullin gene family in teleost fish. / Ogoshi, Maho; Inoue, Koji; Takei, Yoshio.

In: Biochemical and Biophysical Research Communications, Vol. 311, No. 4, 28.11.2003, p. 1072-1077.

Research output: Contribution to journalArticle

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