Identification of a gene encoding a novel thiosulfate:quinone oxidoreductase in marine Acidithiobacillus sp. strain SH

Tadayoshi Kanao, Sultana Sharmin, Mirai Tokuhisa, Maho Otsuki, Kazuo Kamimura

Research output: Contribution to journalArticlepeer-review

Abstract

Sulfur-oxidizing bacteria that are halophilic and acidophilic have gained interest because of their potential use in bioleaching operations in salt-containing environments. Acidithiobacillus sp. strain SH, which was previously identified as Acidithiobacillus thiooxidans based on its 16S rRNA gene sequence, is a chemolithoautotrophic marine bacterium exhibiting sodium chloride-stimulated thiosulfate-oxidizing activities. A novel thiosulfate:quinone oxidoreductase from strain SH (SH-TQO) has been purified from its solubilized membrane fraction. The gene for SH-TQO was determined from the draft genome sequence of the strain SH. Amino acid sequences of peptides generated by the in-gel trypsin digestion of SH-TQO were found in a protein encoded by locus tag B1757_09800 of the genome of the strain SH. The gene encoded 444 amino acids with a signal peptide of 29 amino acids and was annotated to encode a porin. The gene was located in a unique genomic region, not found in A. thiooxidans strains, suggesting that the strain SH acquired this region through a horizontal gene transfer. A protein–protein basic local alignment search revealed that sulfur-oxidizing bacteria, such as Acidithiobacillus species have proteins homologous to SH-TQO, though the degree of homologies was relatively low. The protein, DoxXA, which is homologous to TQO from Acidianus amvibalens, was also found in the genomic region.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalResearch in Microbiology
Volume171
Issue number7
DOIs
Publication statusPublished - Oct 1 2020

Keywords

  • Acidithiobacillus
  • Acidophile
  • Marine sulfur-oxidizer
  • Thiosulfate oxidation
  • Thiosulfate:quinone oxidoreductase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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