Abstract
Among the proteins localized on the cell wall (frustule) of diatoms (frustule-associated proteins), several proteins tightly associated with the cell wall have been implicated in frustule formation. These proteins include diatom-specific unique serine- and lysine-rich sequences represented by silaffins. Taking advantage of available genome information, we used a recently described bioinformatics approach to screen silaffin-like proteins rich in serine and lysine from the genome of the marine pennate diatom Fistulifera sp. strain JPCC DA0580 and identified 7 proteins. All of the proteins shared a sequence motif called the XGXG domain, which was also confirmed in a silaffin-like protein identified in other diatoms. In vivo localization analysis revealed that one of the identified proteins, G7408, occurs throughout the frustule with a slightly uneven distribution. This novel frustule-associated protein could be a useful tool to elucidate the mechanism of biosilica formation in diatoms and to functionalize this strain for future biotechnological applications.
| Original language | English |
|---|---|
| Pages (from-to) | 39-44 |
| Number of pages | 6 |
| Journal | Marine Genomics |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
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Keywords
- Biomineral
- Fistulifera sp. strain JPCC DA0580
- Frustule-associated protein
- Marine diatom
- Whole genome
ASJC Scopus subject areas
- Aquatic Science
- Genetics
Cite this
Identification of a frustule-associated protein of the marine pennate diatom Fistulifera sp. strain JPCC DA0580. / Nemoto, Michiko; Maeda, Yoshiaki; Muto, Masaki; Tanaka, Masayoshi; Yoshino, Tomoko; Mayama, Shigeki; Tanaka, Tsuyoshi.
In: Marine Genomics, Vol. 16, No. 1, 2014, p. 39-44.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a frustule-associated protein of the marine pennate diatom Fistulifera sp. strain JPCC DA0580
AU - Nemoto, Michiko
AU - Maeda, Yoshiaki
AU - Muto, Masaki
AU - Tanaka, Masayoshi
AU - Yoshino, Tomoko
AU - Mayama, Shigeki
AU - Tanaka, Tsuyoshi
PY - 2014
Y1 - 2014
N2 - Among the proteins localized on the cell wall (frustule) of diatoms (frustule-associated proteins), several proteins tightly associated with the cell wall have been implicated in frustule formation. These proteins include diatom-specific unique serine- and lysine-rich sequences represented by silaffins. Taking advantage of available genome information, we used a recently described bioinformatics approach to screen silaffin-like proteins rich in serine and lysine from the genome of the marine pennate diatom Fistulifera sp. strain JPCC DA0580 and identified 7 proteins. All of the proteins shared a sequence motif called the XGXG domain, which was also confirmed in a silaffin-like protein identified in other diatoms. In vivo localization analysis revealed that one of the identified proteins, G7408, occurs throughout the frustule with a slightly uneven distribution. This novel frustule-associated protein could be a useful tool to elucidate the mechanism of biosilica formation in diatoms and to functionalize this strain for future biotechnological applications.
AB - Among the proteins localized on the cell wall (frustule) of diatoms (frustule-associated proteins), several proteins tightly associated with the cell wall have been implicated in frustule formation. These proteins include diatom-specific unique serine- and lysine-rich sequences represented by silaffins. Taking advantage of available genome information, we used a recently described bioinformatics approach to screen silaffin-like proteins rich in serine and lysine from the genome of the marine pennate diatom Fistulifera sp. strain JPCC DA0580 and identified 7 proteins. All of the proteins shared a sequence motif called the XGXG domain, which was also confirmed in a silaffin-like protein identified in other diatoms. In vivo localization analysis revealed that one of the identified proteins, G7408, occurs throughout the frustule with a slightly uneven distribution. This novel frustule-associated protein could be a useful tool to elucidate the mechanism of biosilica formation in diatoms and to functionalize this strain for future biotechnological applications.
KW - Biomineral
KW - Fistulifera sp. strain JPCC DA0580
KW - Frustule-associated protein
KW - Marine diatom
KW - Whole genome
UR - http://www.scopus.com/inward/record.url?scp=84922217575&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922217575&partnerID=8YFLogxK
U2 - 10.1016/j.margen.2014.01.006
DO - 10.1016/j.margen.2014.01.006
M3 - Article
C2 - 24517995
AN - SCOPUS:84922217575
VL - 16
SP - 39
EP - 44
JO - Marine Genomics
JF - Marine Genomics
SN - 1874-7787
IS - 1
ER -