Identification of a frustule-associated protein of the marine pennate diatom Fistulifera sp. strain JPCC DA0580

Michiko Nemoto, Yoshiaki Maeda, Masaki Muto, Masayoshi Tanaka, Tomoko Yoshino, Shigeki Mayama, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Among the proteins localized on the cell wall (frustule) of diatoms (frustule-associated proteins), several proteins tightly associated with the cell wall have been implicated in frustule formation. These proteins include diatom-specific unique serine- and lysine-rich sequences represented by silaffins. Taking advantage of available genome information, we used a recently described bioinformatics approach to screen silaffin-like proteins rich in serine and lysine from the genome of the marine pennate diatom Fistulifera sp. strain JPCC DA0580 and identified 7 proteins. All of the proteins shared a sequence motif called the XGXG domain, which was also confirmed in a silaffin-like protein identified in other diatoms. In vivo localization analysis revealed that one of the identified proteins, G7408, occurs throughout the frustule with a slightly uneven distribution. This novel frustule-associated protein could be a useful tool to elucidate the mechanism of biosilica formation in diatoms and to functionalize this strain for future biotechnological applications.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalMarine Genomics
Volume16
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Diatoms
Bacillariophyceae
diatom
protein
Proteins
proteins
serine
Cell Wall
Serine
lysine
Lysine
cell walls
genome
Genome
bioinformatics
Computational Biology
amino acid sequences

Keywords

  • Biomineral
  • Fistulifera sp. strain JPCC DA0580
  • Frustule-associated protein
  • Marine diatom
  • Whole genome

ASJC Scopus subject areas

  • Aquatic Science
  • Genetics

Cite this

Identification of a frustule-associated protein of the marine pennate diatom Fistulifera sp. strain JPCC DA0580. / Nemoto, Michiko; Maeda, Yoshiaki; Muto, Masaki; Tanaka, Masayoshi; Yoshino, Tomoko; Mayama, Shigeki; Tanaka, Tsuyoshi.

In: Marine Genomics, Vol. 16, No. 1, 2014, p. 39-44.

Research output: Contribution to journalArticle

Nemoto, Michiko ; Maeda, Yoshiaki ; Muto, Masaki ; Tanaka, Masayoshi ; Yoshino, Tomoko ; Mayama, Shigeki ; Tanaka, Tsuyoshi. / Identification of a frustule-associated protein of the marine pennate diatom Fistulifera sp. strain JPCC DA0580. In: Marine Genomics. 2014 ; Vol. 16, No. 1. pp. 39-44.
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