Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein

M. Abe, Taku Takahashi, Y. Komeda

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The Arabidopsis thaliana PROTODERMAL FACTOR1 (PDF1) gene encoding a putative extracellular proline-rich protein is exclusively expressed in the L1 layer of shoot apices and the protoderm of organ primordia. In order to identify essential cis-regulatory sequences required for the L1 layer-specific expression, a series of 5′ deletions of the PDF1 promoter were fused to the β-glucronidase (GUS) gene and introduced into Arabidopsis plants. Our analysis revealed that the minimum region necessary to confer L1-specific expression of PDF1 is confined within a 260-bp fragment upstream of the transcription start site, We identified an 8-bp motif in this region that is conserved between promoter regions of all the L1-specific genes so far cloned, and we designated it the L1 box. Electrophoretic mobility shift assays demonstrated that the L1-specific homeodomain protein ATML1 can bind to the L1 box sequence in vitro. The GUS expression in transgenic plants disappeared when a mutation that abolishes binding of ATML1 was introduced into the PDF1 /1 box sequence of the construct. These results suggest that the L1 box plays a crucial role in the regulation of PDF1 expression in L1 cells and that ATML1 could cooperate to drive L1-specific expression.

Original languageEnglish
Pages (from-to)487-494
Number of pages8
JournalPlant Journal
Volume26
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Long Interspersed Nucleotide Elements
homeodomain proteins
Homeodomain Proteins
regulatory sequences
Gene Expression
Arabidopsis
gene expression
promoter regions
Genes
genes
Transcription Initiation Site
Genetically Modified Plants
Electrophoretic Mobility Shift Assay
Genetic Promoter Regions
Proline
proline
transgenic plants
Arabidopsis thaliana
transcription (genetics)
mutation

Keywords

  • Arabidopsis thaliana
  • Cis-regulatory element
  • L1 box
  • L1 layer
  • PROTODERMAL FACTOR 1

ASJC Scopus subject areas

  • Plant Science

Cite this

Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein. / Abe, M.; Takahashi, Taku; Komeda, Y.

In: Plant Journal, Vol. 26, No. 5, 2001, p. 487-494.

Research output: Contribution to journalArticle

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