Identification of a cis-acting element of ART1, a C2H2-type zinc-finger transcription factor for aluminum tolerance in rice

Tomokazu Tsutsui, Naoki Yamaji, Jian Feng Ma

Research output: Contribution to journalArticle

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Abstract

Rice (Oryza sativa) is one of the most aluminum (Al)-tolerant species among small-grain cereals. Recent identification of a transcription factor AL RESISTANCE TRANSCRIPTION FACTOR1 (ART1) revealed that this high Al tolerance in rice is achieved by multiple genes involved in detoxification of Al at different cellular levels. ART1 is a C2H2-type zinc-finger transcription factor and regulates the expression of 31 genes in the downstream. In this study, we attempted to identify a cisacting element of ART1. We used the promoter region of SENSITIVE TO AL RHIZOTOXICITY1, an Al tolerance gene in the downstream of ART1. With the help of gel-shift assay, we were able to identify the cis-acting element as GGN(T/g/a/C)V(C/ A/g)S(C/G). This element was found in the promoter region of 29 genes among 31 ART1-regulated genes. To confirm this cisacting element in vivo, we transiently introduced this element one or five times tandemly repeated sequence with 35S minimal promoter and green fluorescent protein reporter together with or without ART1 gene in the tobacco (Nicotiana tabacum) mesophyll protoplasts. The results showed that the expression of green fluorescent protein reporter responded to ART1 expression. Furthermore, the expression increased with repetition of the cis-acting element. Our results indicate that the five nucleotides identified are the target DNA-binding sequence of ART1.

Original languageEnglish
Pages (from-to)925-931
Number of pages7
JournalPlant Physiology
Volume156
Issue number2
DOIs
Publication statusPublished - Jun 2011

Fingerprint

zinc finger motif
Aluminum
aluminum
Transcription Factors
transcription factors
transcription (genetics)
rice
Genes
Green Fluorescent Proteins
Genetic Promoter Regions
Tobacco
genes
promoter regions
green fluorescent protein
Protoplasts
Nucleotides
Gels
Oryza
CYS2-HIS2 Zinc Fingers
Gene Expression

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

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Identification of a cis-acting element of ART1, a C2H2-type zinc-finger transcription factor for aluminum tolerance in rice. / Tsutsui, Tomokazu; Yamaji, Naoki; Ma, Jian Feng.

In: Plant Physiology, Vol. 156, No. 2, 06.2011, p. 925-931.

Research output: Contribution to journalArticle

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