Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10

Iichiro Ohhiral; Shinsuke Kuwaki; Hidetoshi Morita; Takehito Suzuki; Satoshi Tomita; Shin Hisamatsu; Shigenori Sonoki; Sumio Shinoda

doi:10.4265/bio.9.77

Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10

Iichiro Ohhiral, Shinsuke Kuwaki, Hidetoshi Morita, Takehito Suzuki, Satoshi Tomita, Shin Hisamatsu, Shigenori Sonoki, Sumio Shinoda

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, "tempeh", and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.

Original language	English
Pages (from-to)	77-81
Number of pages	5
Journal	Biocontrol science
Volume	9
Issue number	3
DOIs	https://doi.org/10.4265/bio.9.77
Publication status	Published - Sep 2004

Keywords

Antibacterial substance
Enterococcus faecalis
Phenyllactic acid

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Public Health, Environmental and Occupational Health

Access to Document

10.4265/bio.9.77

Fingerprint Dive into the research topics of 'Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10'. Together they form a unique fingerprint.

Cite this

Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10. / Ohhiral, Iichiro; Kuwaki, Shinsuke; Morita, Hidetoshi; Suzuki, Takehito; Tomita, Satoshi; Hisamatsu, Shin; Sonoki, Shigenori; Shinoda, Sumio.

In: Biocontrol science, Vol. 9, No. 3, 09.2004, p. 77-81.

Research output: Contribution to journal › Article › peer-review

@article{8f8b6fa86e7641ca84529776e6c444fe,

title = "Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10",

abstract = "Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, {"}tempeh{"}, and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.",

keywords = "Antibacterial substance, Enterococcus faecalis, Phenyllactic acid",

author = "Iichiro Ohhiral and Shinsuke Kuwaki and Hidetoshi Morita and Takehito Suzuki and Satoshi Tomita and Shin Hisamatsu and Shigenori Sonoki and Sumio Shinoda",

year = "2004",

month = sep,

doi = "10.4265/bio.9.77",

language = "English",

volume = "9",

pages = "77--81",

journal = "Biocontrol Science",

issn = "1342-4815",

publisher = "Society for Antibacterial and Antifungal Agents Japan",

number = "3",

}

TY - JOUR

T1 - Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10

AU - Ohhiral, Iichiro

AU - Kuwaki, Shinsuke

AU - Morita, Hidetoshi

AU - Suzuki, Takehito

AU - Tomita, Satoshi

AU - Hisamatsu, Shin

AU - Sonoki, Shigenori

AU - Shinoda, Sumio

PY - 2004/9

Y1 - 2004/9

N2 - Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, "tempeh", and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.

AB - Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, "tempeh", and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.

KW - Antibacterial substance

KW - Enterococcus faecalis

KW - Phenyllactic acid

UR - http://www.scopus.com/inward/record.url?scp=7044268944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7044268944&partnerID=8YFLogxK

U2 - 10.4265/bio.9.77

DO - 10.4265/bio.9.77

M3 - Article

AN - SCOPUS:7044268944

VL - 9

SP - 77

EP - 81

JO - Biocontrol Science

JF - Biocontrol Science

SN - 1342-4815

IS - 3

ER -