Abstract
Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, "tempeh", and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.
| Original language | English |
|---|---|
| Pages (from-to) | 77-81 |
| Number of pages | 5 |
| Journal | Biocontrol Science |
| Volume | 9 |
| Issue number | 3 |
| Publication status | Published - Sep 2004 |
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Keywords
- Antibacterial substance
- Enterococcus faecalis
- Phenyllactic acid
ASJC Scopus subject areas
- Environmental Engineering
Cite this
Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10. / Ohhiral, Iichiro; Kuwaki, Shinsuke; Morita, Hidetoshi; Suzuki, Takehito; Tomita, Satoshi; Hisamatsu, Shin; Sonoki, Shigenori; Shinoda, Sumio.
In: Biocontrol Science, Vol. 9, No. 3, 09.2004, p. 77-81.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10
AU - Ohhiral, Iichiro
AU - Kuwaki, Shinsuke
AU - Morita, Hidetoshi
AU - Suzuki, Takehito
AU - Tomita, Satoshi
AU - Hisamatsu, Shin
AU - Sonoki, Shigenori
AU - Shinoda, Sumio
PY - 2004/9
Y1 - 2004/9
N2 - Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, "tempeh", and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.
AB - Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, "tempeh", and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.
KW - Antibacterial substance
KW - Enterococcus faecalis
KW - Phenyllactic acid
UR - http://www.scopus.com/inward/record.url?scp=7044268944&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7044268944&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:7044268944
VL - 9
SP - 77
EP - 81
JO - Biocontrol Science
JF - Biocontrol Science
SN - 1342-4815
IS - 3
ER -