Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10

Iichiro Ohhiral, Shinsuke Kuwaki, Hidetoshi Morita, Takehito Suzuki, Satoshi Tomita, Shin Hisamatsu, Shigenori Sonoki, Sumio Shinoda

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Enterococcus faecalls TH10 is a lactic acid bacterial strain isolated from the Malaysian traditional fermented food, "tempeh", and has antibacterial activity against various pathogens. To identify the antibacterial substance, the butanol extract of the culture supernatant of E. faecalls TH10 was fractionated by HPLC equipped with a reversed-phase partition column, and the elutes were subjected to antibacterial assay. As the activity was observed In a fraction eluted by ca 80% methanol, the fraction was analyzed by gas chromatography/mass spectrometry and 3-phenyllactic acid was identified as the major compound. Fractionation with an optical isomer separation column showed that the preparation contained D- and L-forms of 3-phenyllactic acid at a ratio of 2:1. Authentic 3-phenyllactic acid showed antibacterial activity against various bacteria such as Staphylococcus aureus and Escherichia coli. These results suggest the possibility that 3-phenyllactic acid is a biopreservative.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalBiocontrol science
Volume9
Issue number3
DOIs
Publication statusPublished - Sep 2004

Keywords

  • Antibacterial substance
  • Enterococcus faecalis
  • Phenyllactic acid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

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