Identification of β1,3-galactosyltransferases responsible for biosynthesis of insect complex-type N-glycans containing a T-antigen unit in the honeybee

Tomomi Ichimiya, Megumi Maeda, Shou Sakamura, Masato Kanazawa, Shoko Nishihara, Yoshinobu Kimura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Honeybees (Apis mellifera) produce unique complex-type N-glycans bearing a Galβ1-3GalNAc (T-antigen) unit, and honeybee-specific N-glycans are linked to royal jelly glycoproteins. In this study, we identified two novel honeybee β1,3-galactosyltransferase (β1,3-GalT) genes responsible for biosynthesis of the T-antigen in insect N-glycans. The products of the two putative β1,3-GalT genes (β1,3-GalT1 and β1,3-GalT2), which were expressed in Sf21 insect cells, transferred galactose (Gal) residues to GalNAc2GlcNAc2Man3GlcNAc2-PA to form the Galβ1-3GalNAc unit, indicating that the identified genes were involved in biosynthesis of the β1-3 Gal-containing N-glycan. Therefore, using biochemistry and molecular biology techniques, we revealed a unique N-glycan biosynthesis mechanism in the cephalic region of honeybees, which has not previously been found in other animal or plant cells.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalGlycoconjugate Journal
Volume32
Issue number3-4
DOIs
Publication statusPublished - May 1 2015

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Galactosyltransferases
Viral Tumor Antigens
Biosynthesis
Polysaccharides
Insects
Genes
Galactose
Bearings (structural)
Sf9 Cells
Biochemistry
Molecular biology
Bees
Plant Cells
Molecular Biology
Glycoproteins
Animals
Head

Keywords

  • Apis mellifera
  • Insect N-glycan
  • Insect β1,3-galactosyltransferase
  • N-glycan biosynthesis
  • Thomsen-Friedenreich antigen

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Identification of β1,3-galactosyltransferases responsible for biosynthesis of insect complex-type N-glycans containing a T-antigen unit in the honeybee. / Ichimiya, Tomomi; Maeda, Megumi; Sakamura, Shou; Kanazawa, Masato; Nishihara, Shoko; Kimura, Yoshinobu.

In: Glycoconjugate Journal, Vol. 32, No. 3-4, 01.05.2015, p. 141-151.

Research output: Contribution to journalArticle

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