Identification by PCR of receptor-like protein kinases from Arabidopsis flowers

Taku Takahashi, Jing Hong Mu, Alexander Gasch, Nam Hai Chua

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have isolated three receptor-like kinase cDNAs from an Arabidopsis flower cDNA library by PCR using degenerate oligonucleotide primers for conserved domains of protein kinases. Cloning and sequencing of the full-length cDNAs, designated RKF1 to 3 (receptor-like kinase in flowers), showed that the putative extracellular domain of the RKF1 protein contains 13 tandem repeats of leucine-rich sequences and those of RKF2 and RKF3 have no significant homology with other plant sequences. RNA blot analysis revealed that the RKF1 mRNA is highly expressed in stamens while RKF2 and RKF3 mRNAs are present at low levels in all organs examined. In situ localization experiments indicated that the RKF1 mRNA is detectable in early flower primordia and during stamen development. In addition, when fused to a GUS reporter gene, the RKF1 promoter directed high GUS expression in pollen grains. Recombinant RKF1, produced in Escherichia coli, was found to have kinase activity with serine/threonine specificity in vitro.

Original languageEnglish
Pages (from-to)587-596
Number of pages10
JournalPlant Molecular Biology
Volume37
Issue number4
DOIs
Publication statusPublished - Jul 1998
Externally publishedYes

Fingerprint

Arabidopsis
protein kinases
Protein Kinases
phosphotransferases (kinases)
Phosphotransferases
stamens
flowers
Polymerase Chain Reaction
Messenger RNA
receptors
Complementary DNA
flower primordia
Tandem Repeat Sequences
DNA Primers
tandem repeat sequences
Cloning
DNA primers
Threonine
Pollen
Gene Library

Keywords

  • Arabidopsis thaliana
  • Gene expression
  • Leucine-rich repeat
  • PCR
  • Receptor-like kinase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Identification by PCR of receptor-like protein kinases from Arabidopsis flowers. / Takahashi, Taku; Mu, Jing Hong; Gasch, Alexander; Chua, Nam Hai.

In: Plant Molecular Biology, Vol. 37, No. 4, 07.1998, p. 587-596.

Research output: Contribution to journalArticle

Takahashi, Taku ; Mu, Jing Hong ; Gasch, Alexander ; Chua, Nam Hai. / Identification by PCR of receptor-like protein kinases from Arabidopsis flowers. In: Plant Molecular Biology. 1998 ; Vol. 37, No. 4. pp. 587-596.
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