Identification and purification of a Bombyx mori homologue of FTZ-F1

Hitoshi Ueda, Susumu Hirose

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mon contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophila melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same Chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B.mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.

Original languageEnglish
Pages (from-to)7229-7234
Number of pages6
JournalNucleic acids research.
Volume18
Issue number24
Publication statusPublished - Dec 25 1990
Externally publishedYes

Fingerprint

Bombyx
Silk
Purification
DNA
Morus
Methylation
Drosophila melanogaster
Digestion
Peptide Hydrolases
Embryonic Structures
Genes
Proteins
Protease
Drosophilidae
Embryo
Regulator
Interference
Gene
Protein
Factors

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Identification and purification of a Bombyx mori homologue of FTZ-F1. / Ueda, Hitoshi; Hirose, Susumu.

In: Nucleic acids research., Vol. 18, No. 24, 25.12.1990, p. 7229-7234.

Research output: Contribution to journalArticle

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