Identification and punctate nuclear localization of a novel noncoding RNA, Ks-1, from the honeybee brain

Miyuki Sawata, Daisuke Yoshino, Hideaki Takeuchi, Azusa Kamikouchi, Kazuaki Ohashi, Takeo Kubo

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We identified a novel gene, Ks-1, which is expressed preferentially in the small-type Kenyon cells of the honeybee brain. This gene is also expressed in some of the large soma neurons in the brain and in the suboesophageal ganglion. Reverse transcription-polymerase chain reaction experiments indicated that Ks-1 transcripts are enriched in the honeybee brain. cDNA cloning revealed that the consensus Ks-1 cDNA is over 17 kbp and contains no significant open reading frames. Furthermore, fluorescent in situ hybridization revealed that Ks-1 transcripts are located in the nuclei of the neural cells, accumulating in some scattered spots. These findings demonstrate that Ks-1 encodes a novel class of noncoding nuclear RNA and is possibly involved in the regulation of neural functions.

Original languageEnglish
Pages (from-to)772-785
Number of pages14
JournalRNA
Volume8
Issue number6
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Kenyon cells
  • Mushroom bodies
  • Neuron
  • Nuclear RNA

ASJC Scopus subject areas

  • Molecular Biology

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