Identification and expression of the genes encoding a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase

Takamasa Tobimatsu, Hideki Kajiura, Michio Yunoki, Muneaki Azuma, Tetsuo Toraya

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Adenosylcobalamin-dependent glycerol dehydratase undergoes inactivation by glycerol, the physiological substrate, during catalysis. In permeabilized cells of Klebsiella pneumoniae, the inactivated enzyme is reactivated in the presence of ATP, Mg2+, and adenosylcobalamin. We identified the two open reading frames as the genes for a reactivating factor for glycerol dehydratase and designated them gdrA and gdrB. The reactivation of the inactivated glycerol dehydratase by the gene products was confirmed in permeabilized recombinant Escherichia coli cells coexpressing GdrA and GdrB proteins with glycerol dehydratase.

Original languageEnglish
Pages (from-to)4110-4113
Number of pages4
JournalJournal of Bacteriology
Volume181
Issue number13
Publication statusPublished - Jul 1999

Fingerprint

glycerol dehydratase
Gene Expression
Klebsiella pneumoniae
Catalysis
Glycerol
Open Reading Frames
Genes
Adenosine Triphosphate
Escherichia coli
cobamamide
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Identification and expression of the genes encoding a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase. / Tobimatsu, Takamasa; Kajiura, Hideki; Yunoki, Michio; Azuma, Muneaki; Toraya, Tetsuo.

In: Journal of Bacteriology, Vol. 181, No. 13, 07.1999, p. 4110-4113.

Research output: Contribution to journalArticle

Tobimatsu, Takamasa ; Kajiura, Hideki ; Yunoki, Michio ; Azuma, Muneaki ; Toraya, Tetsuo. / Identification and expression of the genes encoding a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 13. pp. 4110-4113.
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