Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds

S. A. Omar, N. I. Elsheery, H. M. Kalaji, M. K.H. Ebrahim, S. Pietkiewicz, C. H. Lee, Suleyman Allakhverdiev, Zeng Fu Xu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y 3SK2 and JcDHN-2 is classified as type Y 2SK2 according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.

Original languageEnglish
Pages (from-to)485-495
Number of pages11
JournalBiochemistry (Moscow)
Volume78
Issue number5
DOIs
Publication statusPublished - May 1 2013
Externally publishedYes

Fingerprint

Jatropha
Seed
Seeds
Complementary DNA
Dehydration
Open Reading Frames
Shorthand
Peptides
Proteins
Cytoprotection
Glycoside Hydrolases
Isoelectric Point
Gene expression
Water content
Real-Time Polymerase Chain Reaction
Gene Expression
Water

Keywords

  • dehydrin
  • Jatropha curcas
  • real time PCR
  • seed development

ASJC Scopus subject areas

  • Biochemistry

Cite this

Omar, S. A., Elsheery, N. I., Kalaji, H. M., Ebrahim, M. K. H., Pietkiewicz, S., Lee, C. H., ... Xu, Z. F. (2013). Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds. Biochemistry (Moscow), 78(5), 485-495. https://doi.org/10.1134/S0006297913050076

Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds. / Omar, S. A.; Elsheery, N. I.; Kalaji, H. M.; Ebrahim, M. K.H.; Pietkiewicz, S.; Lee, C. H.; Allakhverdiev, Suleyman; Xu, Zeng Fu.

In: Biochemistry (Moscow), Vol. 78, No. 5, 01.05.2013, p. 485-495.

Research output: Contribution to journalArticle

Omar, SA, Elsheery, NI, Kalaji, HM, Ebrahim, MKH, Pietkiewicz, S, Lee, CH, Allakhverdiev, S & Xu, ZF 2013, 'Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds', Biochemistry (Moscow), vol. 78, no. 5, pp. 485-495. https://doi.org/10.1134/S0006297913050076
Omar, S. A. ; Elsheery, N. I. ; Kalaji, H. M. ; Ebrahim, M. K.H. ; Pietkiewicz, S. ; Lee, C. H. ; Allakhverdiev, Suleyman ; Xu, Zeng Fu. / Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds. In: Biochemistry (Moscow). 2013 ; Vol. 78, No. 5. pp. 485-495.
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abstract = "Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y 3SK2 and JcDHN-2 is classified as type Y 2SK2 according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42{\%} in mature seeds to 12{\%} in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.",
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