Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds

S. A. Omar, N. I. Elsheery, H. M. Kalaji, M. K.H. Ebrahim, S. Pietkiewicz, C. H. Lee, S. I. Allakhverdiev, Zeng Fu Xu

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y 3SK2 and JcDHN-2 is classified as type Y 2SK2 according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.

    Original languageEnglish
    Pages (from-to)485-495
    Number of pages11
    JournalBiochemistry (Moscow)
    Volume78
    Issue number5
    DOIs
    Publication statusPublished - May 2013

    Keywords

    • Jatropha curcas
    • dehydrin
    • real time PCR
    • seed development

    ASJC Scopus subject areas

    • Biochemistry

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