Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells

Ai Ping Wang, Akihiro Wada, Kinnosuke Yahiro, Tomohiko Nomura, Yoshio Fujii, Keinosuke Okamoto, Youhei Mizuta, Shigeru Kohno, Joel Moss, Toshiya Hirayama

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. By immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kDa protein (p66) was identified as a receptor for A. sobria hemolysin on Intestine 407 cells. Treatment of p66 with N-glycosidase F reduced the apparent sized of p66 to 60 kDa on SDS-polyacrylamide gels. p66, released from Intestine 407 cells following incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) treatment, bound A. sobria hemolysin. Thus treatment of Intestine 407 cells with PI-PLC resulted in the remarkable decrease of the sensitivity to A. sobria hemolysin. These results are consistent with the hypothesis that p66, the binding protein for A. sobria hemolysin, is a glycosylphosphatidylinositol-anchored glycoprotein expressed on the surface of Intestine 407 cells and probably plays a role as a receptor for A. sobria hemolysin on the intestinal cells.

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalMicrobial Pathogenesis
Volume27
Issue number4
DOIs
Publication statusPublished - Oct 1999
Externally publishedYes

Fingerprint

Aeromonas
Hemolysin Proteins
Intestines
Glycoproteins
Phosphoinositide Phospholipase C
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Glycosylphosphatidylinositols
Staphylococcal Protein A
Immunoprecipitation
Anti-Idiotypic Antibodies
Diarrhea
Carrier Proteins
Bacteria

Keywords

  • Aeromonas sobria
  • Glycoprotein receptor
  • Hemolysin
  • Toxin action
  • Toxin receptor

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells. / Wang, Ai Ping; Wada, Akihiro; Yahiro, Kinnosuke; Nomura, Tomohiko; Fujii, Yoshio; Okamoto, Keinosuke; Mizuta, Youhei; Kohno, Shigeru; Moss, Joel; Hirayama, Toshiya.

In: Microbial Pathogenesis, Vol. 27, No. 4, 10.1999, p. 215-221.

Research output: Contribution to journalArticle

Wang, AP, Wada, A, Yahiro, K, Nomura, T, Fujii, Y, Okamoto, K, Mizuta, Y, Kohno, S, Moss, J & Hirayama, T 1999, 'Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells', Microbial Pathogenesis, vol. 27, no. 4, pp. 215-221. https://doi.org/10.1006/mpat.1999.0299
Wang, Ai Ping ; Wada, Akihiro ; Yahiro, Kinnosuke ; Nomura, Tomohiko ; Fujii, Yoshio ; Okamoto, Keinosuke ; Mizuta, Youhei ; Kohno, Shigeru ; Moss, Joel ; Hirayama, Toshiya. / Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells. In: Microbial Pathogenesis. 1999 ; Vol. 27, No. 4. pp. 215-221.
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