Identification and characterization of rice oshkt1;3 variants

Shahin Imran, Yoshiyuki Tsuchiya, Sen Thi Huong Tran, Maki Katsuhara

Research output: Contribution to journalArticlepeer-review

Abstract

In rice, the high-affinity K+ transporter, OsHKT1;3, functions as a Na+-selective transporter. mRNA variants of OsHKT1;3 have been reported previously, but their functions remain unknown. In this study, five OsHKT1;3 variants (V1-V5) were identified from japonica rice (Nipponbare) in addition to OsHKT1;3_FL. Absolute quantification qPCR analyses revealed that the transcript level of OsHKT1;3_FL was significantly higher than other variants in both the roots and shoots. Expression levels of OsHKT1;3_FL, and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using Xenopus laevis oocytes revealed that oocytes expressing OsHKT1;3_FL and all of its variants exhibited smaller Na+ currents. The presented data, together with previous data, provide insights to understanding how OsHKT family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice.

Original languageEnglish
Article number2006
JournalPlants
Volume10
Issue number10
DOIs
Publication statusPublished - Oct 2021

Keywords

  • 3
  • MRNA variants
  • Na transport
  • OsHKT1
  • Rice
  • TEVC

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

Fingerprint

Dive into the research topics of 'Identification and characterization of rice oshkt1;3 variants'. Together they form a unique fingerprint.

Cite this