Identification and characterization of mouse SSX genes: A multigene family on the X chromosome with restricted cancer/testis expression

Yao Tseng Chen, Birgit Alpen, Toshiro Ono, Ali O. Gure, Matthew A. Scanlan, William H. Biggs, Karen Arden, Eiichi Nakayama, Lloyd J. Old

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20 Citations (Scopus)

Abstract

Human SSX was first identified as the gene involved in the t(X;18) translocation in synovial sarcoma. SSX is a multigene family, with 9 complete genes on chromosome Xp11. Normally expressed almost exclusively in testis, SSX mRNA is expressed in various human tumors, defining SSX as a cancer/testis antigen. We have now cloned the mouse ortholog of SSX. Mouse SSX genes can be divided into Ssxa and Ssxb subfamilies based on sequence homology. Ssxa has only one member, whereas 12 Ssxb genes, Ssxb1 to Ssxb12, were identified by cDNA cloning from mouse testis and mouse tumors. Both Ssxa and Ssxb are located on chromosome X and show tissue-restricted mRNA expression to testis among normal tissues. All putative human and mouse SSX proteins share conserved KRAB and SSX-RD domains. Mouse tumors were found to express some, but not all, Ssxb genes, similar to the SSX activation in human tumors.

Original languageEnglish
Pages (from-to)628-636
Number of pages9
JournalGenomics
Volume82
Issue number6
DOIs
Publication statusPublished - Dec 2003

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Keywords

  • Cancer vaccine
  • Cancer/testis antigens
  • Tumor immunology

ASJC Scopus subject areas

  • Genetics

Cite this

Chen, Y. T., Alpen, B., Ono, T., Gure, A. O., Scanlan, M. A., Biggs, W. H., Arden, K., Nakayama, E., & Old, L. J. (2003). Identification and characterization of mouse SSX genes: A multigene family on the X chromosome with restricted cancer/testis expression. Genomics, 82(6), 628-636. https://doi.org/10.1016/S0888-7543(03)00183-6