Identification and characterization of mammalian 5-formyluracil-DNA glycosylase.

Mayumi Matsubara, Aya Masaoka, Tamon Tanaka, Hiroaki Terato, Yoshihiko Ohyama, Hiroshi Ide

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

5-Formyluracil is a major oxidative thymine lesion with mutagenic and cytotoxic properties. In this study, we have partially purified and characterized a mammalian 5-formyluracil-DNA glycosylase (FDG) from rat liver. FDG was a monofunctional DNA glycosylase and removed 5-formyluracil, uracil, 5-hydroxyuracil, 5-hydroxylmethyluracil in single-stranded and double-stranded DNA. Several lines of evidence indicate that FDG is a rat SMUG1 homologue. Human SMUG1 also exhibited similar enzymatic properties.

Original languageEnglish
Pages (from-to)233-234
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number3
Publication statusPublished - Jan 1 2003
Externally publishedYes

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DNA Glycosylases
Thymine
Uracil
5-formyluracil
Liver
DNA

Cite this

Identification and characterization of mammalian 5-formyluracil-DNA glycosylase. / Matsubara, Mayumi; Masaoka, Aya; Tanaka, Tamon; Terato, Hiroaki; Ohyama, Yoshihiko; Ide, Hiroshi.

In: Nucleic acids research. Supplement (2001), No. 3, 01.01.2003, p. 233-234.

Research output: Contribution to journalArticle

Matsubara, Mayumi ; Masaoka, Aya ; Tanaka, Tamon ; Terato, Hiroaki ; Ohyama, Yoshihiko ; Ide, Hiroshi. / Identification and characterization of mammalian 5-formyluracil-DNA glycosylase. In: Nucleic acids research. Supplement (2001). 2003 ; No. 3. pp. 233-234.
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