Identification and characterization of a novel progesterone receptor-binding element in the mouse prostaglandin E receptor subtype EP2 gene

Sohken Tsuchiya, Satoshi Tanaka, Yukihiko Sugimoto, Masato Katsuyama, Reiko Ikegami, Atsushi Ichikawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Gene expression of prostaglandin E receptor EP2 is induced in the luminal epithelium of the mouse uterus during peri-implantation period (day-5 of pseudopregnancy), suggesting the involvement of progesterone and its receptor (PR) in this expression. However it remains unclear whether PR affects EP2 gene expression through its binding. Results: We investigated transcriptional regulation of EP2 gene expression with reporter gene analysis using HeLa cells with or without expression of the PR. The 5′-flanking region (-3260 to -27, upstream of the translation initiation site) exhibited progesterone-induced promoter activation and basal promoter activity in the presence of PR. Using successive deletion analysis, we determined the six regulatory regions in the EP2 gene. Three regions were found to be involved in progesterone-induced promoter activation, whereas the other three regions were involved in basal promoter activity in the presence of PR. We identified a novel PR-binding sequence, 5′-G(G/A)CCGGA-3′, in the two basal promoter regions and Sp1- and Sp3-binding in the other basal promoter region. Conclusions: We identified a novel PR-binding sequence, which may be involved in the regulation of basal promoter activity in the EP2 gene.

Original languageEnglish
Pages (from-to)747-758
Number of pages12
JournalGenes to Cells
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 1 2003
Externally publishedYes

Fingerprint

Receptors, Prostaglandin E, EP2 Subtype
Progesterone Receptors
Genetic Promoter Regions
Progesterone
Prostaglandin E Receptors
Pseudopregnancy
Gene Expression
5' Flanking Region
Nucleic Acid Regulatory Sequences
Gene Expression Regulation
Reporter Genes
HeLa Cells
Genes
Uterus
Epithelium

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Identification and characterization of a novel progesterone receptor-binding element in the mouse prostaglandin E receptor subtype EP2 gene. / Tsuchiya, Sohken; Tanaka, Satoshi; Sugimoto, Yukihiko; Katsuyama, Masato; Ikegami, Reiko; Ichikawa, Atsushi.

In: Genes to Cells, Vol. 8, No. 9, 01.09.2003, p. 747-758.

Research output: Contribution to journalArticle

Tsuchiya, Sohken ; Tanaka, Satoshi ; Sugimoto, Yukihiko ; Katsuyama, Masato ; Ikegami, Reiko ; Ichikawa, Atsushi. / Identification and characterization of a novel progesterone receptor-binding element in the mouse prostaglandin E receptor subtype EP2 gene. In: Genes to Cells. 2003 ; Vol. 8, No. 9. pp. 747-758.
@article{1ce44f876584473a9c3a99ddf8c7b3ec,
title = "Identification and characterization of a novel progesterone receptor-binding element in the mouse prostaglandin E receptor subtype EP2 gene",
abstract = "Background: Gene expression of prostaglandin E receptor EP2 is induced in the luminal epithelium of the mouse uterus during peri-implantation period (day-5 of pseudopregnancy), suggesting the involvement of progesterone and its receptor (PR) in this expression. However it remains unclear whether PR affects EP2 gene expression through its binding. Results: We investigated transcriptional regulation of EP2 gene expression with reporter gene analysis using HeLa cells with or without expression of the PR. The 5′-flanking region (-3260 to -27, upstream of the translation initiation site) exhibited progesterone-induced promoter activation and basal promoter activity in the presence of PR. Using successive deletion analysis, we determined the six regulatory regions in the EP2 gene. Three regions were found to be involved in progesterone-induced promoter activation, whereas the other three regions were involved in basal promoter activity in the presence of PR. We identified a novel PR-binding sequence, 5′-G(G/A)CCGGA-3′, in the two basal promoter regions and Sp1- and Sp3-binding in the other basal promoter region. Conclusions: We identified a novel PR-binding sequence, which may be involved in the regulation of basal promoter activity in the EP2 gene.",
author = "Sohken Tsuchiya and Satoshi Tanaka and Yukihiko Sugimoto and Masato Katsuyama and Reiko Ikegami and Atsushi Ichikawa",
year = "2003",
month = "9",
day = "1",
doi = "10.1046/j.1365-2443.2003.00672.x",
language = "English",
volume = "8",
pages = "747--758",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Identification and characterization of a novel progesterone receptor-binding element in the mouse prostaglandin E receptor subtype EP2 gene

AU - Tsuchiya, Sohken

AU - Tanaka, Satoshi

AU - Sugimoto, Yukihiko

AU - Katsuyama, Masato

AU - Ikegami, Reiko

AU - Ichikawa, Atsushi

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Background: Gene expression of prostaglandin E receptor EP2 is induced in the luminal epithelium of the mouse uterus during peri-implantation period (day-5 of pseudopregnancy), suggesting the involvement of progesterone and its receptor (PR) in this expression. However it remains unclear whether PR affects EP2 gene expression through its binding. Results: We investigated transcriptional regulation of EP2 gene expression with reporter gene analysis using HeLa cells with or without expression of the PR. The 5′-flanking region (-3260 to -27, upstream of the translation initiation site) exhibited progesterone-induced promoter activation and basal promoter activity in the presence of PR. Using successive deletion analysis, we determined the six regulatory regions in the EP2 gene. Three regions were found to be involved in progesterone-induced promoter activation, whereas the other three regions were involved in basal promoter activity in the presence of PR. We identified a novel PR-binding sequence, 5′-G(G/A)CCGGA-3′, in the two basal promoter regions and Sp1- and Sp3-binding in the other basal promoter region. Conclusions: We identified a novel PR-binding sequence, which may be involved in the regulation of basal promoter activity in the EP2 gene.

AB - Background: Gene expression of prostaglandin E receptor EP2 is induced in the luminal epithelium of the mouse uterus during peri-implantation period (day-5 of pseudopregnancy), suggesting the involvement of progesterone and its receptor (PR) in this expression. However it remains unclear whether PR affects EP2 gene expression through its binding. Results: We investigated transcriptional regulation of EP2 gene expression with reporter gene analysis using HeLa cells with or without expression of the PR. The 5′-flanking region (-3260 to -27, upstream of the translation initiation site) exhibited progesterone-induced promoter activation and basal promoter activity in the presence of PR. Using successive deletion analysis, we determined the six regulatory regions in the EP2 gene. Three regions were found to be involved in progesterone-induced promoter activation, whereas the other three regions were involved in basal promoter activity in the presence of PR. We identified a novel PR-binding sequence, 5′-G(G/A)CCGGA-3′, in the two basal promoter regions and Sp1- and Sp3-binding in the other basal promoter region. Conclusions: We identified a novel PR-binding sequence, which may be involved in the regulation of basal promoter activity in the EP2 gene.

UR - http://www.scopus.com/inward/record.url?scp=0141747238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141747238&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2443.2003.00672.x

DO - 10.1046/j.1365-2443.2003.00672.x

M3 - Article

C2 - 12940822

AN - SCOPUS:0141747238

VL - 8

SP - 747

EP - 758

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 9

ER -