Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans

R. Nomura, K. Nakano, Shuhei Naka, H. Nemoto, K. Masuda, J. Lapirattanakul, S. Alaluusua, Michiyo Nakano, S. Kawabata, T. Ooshima

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Streptococcus mutans, a major pathogen of dental caries, is occasionally isolated from the blood of patients with infective endocarditis. Bacterial attachment of exposed collagen tissue in the impaired endothelium is an important step in the onset of infective endocarditis. In our previous studies, some S. mutans strains were shown to possess collagen-binding activities and most of them had an approximately 120-kDa cell-surface collagen-binding protein called Cnm. However, several strains without Cnm proteins show collagen-binding properties. In the present study, another collagen-binding protein, Cbm, was characterized and its coding gene cbm was sequenced in these strains. The amino acid alignment in the putative collagen-binding domain of Cbm was shown to have approximately 80% identity and 90% similarity to the comparable region of Cnm. Cbm-deficient isogenic mutant strains constructed by insertional inactivation of the cbm gene, lacked collagen-binding properties, which were recovered in the complemented mutant. Analyses of a large number of clinical isolates from Japan, Thailand and Finland revealed that cbm-positive strains were present in all of these countries and that cnm-positive and cbm-positive strains were detected in the oral cavity of approximately 10 and 2% of systemically healthy subjects, respectively. In addition, cnm-positive strains were predominantly identified in the serotype f group, whereas cbm-positive strains were frequently detected in serotype k. These results suggest that Cbm as well as Cnm are major cell surface proteins of S. mutans associated with binding to type I collagen and predominantly identified in serotype k strains.

Original languageEnglish
Pages (from-to)308-323
Number of pages16
JournalMolecular Oral Microbiology
Volume27
Issue number4
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

Fingerprint

Streptococcus mutans
Carrier Proteins
Collagen
Endocarditis
Protein S
Dental Caries
Gene Silencing
Thailand
Finland
Collagen Type I
Endothelium
Mouth
Healthy Volunteers
Japan
Membrane Proteins
Amino Acids
Genes
Serogroup

Keywords

  • Collagen-binding protein
  • Infective endocarditis
  • Serotype
  • Streptococcus mutans

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans. / Nomura, R.; Nakano, K.; Naka, Shuhei; Nemoto, H.; Masuda, K.; Lapirattanakul, J.; Alaluusua, S.; Nakano, Michiyo; Kawabata, S.; Ooshima, T.

In: Molecular Oral Microbiology, Vol. 27, No. 4, 08.2012, p. 308-323.

Research output: Contribution to journalArticle

Nomura, R, Nakano, K, Naka, S, Nemoto, H, Masuda, K, Lapirattanakul, J, Alaluusua, S, Nakano, M, Kawabata, S & Ooshima, T 2012, 'Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans', Molecular Oral Microbiology, vol. 27, no. 4, pp. 308-323. https://doi.org/10.1111/j.2041-1014.2012.00649.x
Nomura, R. ; Nakano, K. ; Naka, Shuhei ; Nemoto, H. ; Masuda, K. ; Lapirattanakul, J. ; Alaluusua, S. ; Nakano, Michiyo ; Kawabata, S. ; Ooshima, T. / Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans. In: Molecular Oral Microbiology. 2012 ; Vol. 27, No. 4. pp. 308-323.
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