Abstract
S100A6 is a Ca2+-signal transducer that interacts with numerous proteins and regulates their biochemical functions. Here we identified a centrosomal protein, FOR20 (FOP-related protein of 20 kDa) as a novel S100A6 target by screening protein microarrays carrying 19,676 recombinant GST-fused human proteins. Binding experiments revealed that S100A6 interacts with the N-terminal region (residues 1–30) of FOR20 in a Ca2+-dependent manner in vitro and in living cells. Several S100 proteins including S100A1, A2, A4, A11, B also exhibited Ca2+-dependent interactions with FOR20 as well as S100A6. We found that two distantly related centrosomal proteins, FOP and OFD1, also possess N-terminal regions with a significant sequence similarity to the putative S100A6-binding site (residues 1–30) in FOR20 and are capable of binding to S100A6 in a Ca2+-dependent manner. Taken together, these results may indicate that S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca2+-dependent regulation of centrosomal function.
| Original language | English |
|---|---|
| Pages (from-to) | 980-985 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 491 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Sep 30 2017 |
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Keywords
- Ca-signaling
- FOR20
- Interactome analysis
- Protein array
- S100A6
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Identification and characterization of a centrosomal protein, FOR20 as a novel S100A6 target. / Sakane, Kyohei; Nishiguchi, Miyu; Denda, Miwako; Yamagchi, Fuminori; Magari, Masaki; Kanayama, Naoki; Morishita, Ryo; Tokumitsu, Hiroshi.
In: Biochemical and Biophysical Research Communications, Vol. 491, No. 4, 30.09.2017, p. 980-985.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and characterization of a centrosomal protein, FOR20 as a novel S100A6 target
AU - Sakane, Kyohei
AU - Nishiguchi, Miyu
AU - Denda, Miwako
AU - Yamagchi, Fuminori
AU - Magari, Masaki
AU - Kanayama, Naoki
AU - Morishita, Ryo
AU - Tokumitsu, Hiroshi
PY - 2017/9/30
Y1 - 2017/9/30
N2 - S100A6 is a Ca2+-signal transducer that interacts with numerous proteins and regulates their biochemical functions. Here we identified a centrosomal protein, FOR20 (FOP-related protein of 20 kDa) as a novel S100A6 target by screening protein microarrays carrying 19,676 recombinant GST-fused human proteins. Binding experiments revealed that S100A6 interacts with the N-terminal region (residues 1–30) of FOR20 in a Ca2+-dependent manner in vitro and in living cells. Several S100 proteins including S100A1, A2, A4, A11, B also exhibited Ca2+-dependent interactions with FOR20 as well as S100A6. We found that two distantly related centrosomal proteins, FOP and OFD1, also possess N-terminal regions with a significant sequence similarity to the putative S100A6-binding site (residues 1–30) in FOR20 and are capable of binding to S100A6 in a Ca2+-dependent manner. Taken together, these results may indicate that S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca2+-dependent regulation of centrosomal function.
AB - S100A6 is a Ca2+-signal transducer that interacts with numerous proteins and regulates their biochemical functions. Here we identified a centrosomal protein, FOR20 (FOP-related protein of 20 kDa) as a novel S100A6 target by screening protein microarrays carrying 19,676 recombinant GST-fused human proteins. Binding experiments revealed that S100A6 interacts with the N-terminal region (residues 1–30) of FOR20 in a Ca2+-dependent manner in vitro and in living cells. Several S100 proteins including S100A1, A2, A4, A11, B also exhibited Ca2+-dependent interactions with FOR20 as well as S100A6. We found that two distantly related centrosomal proteins, FOP and OFD1, also possess N-terminal regions with a significant sequence similarity to the putative S100A6-binding site (residues 1–30) in FOR20 and are capable of binding to S100A6 in a Ca2+-dependent manner. Taken together, these results may indicate that S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca2+-dependent regulation of centrosomal function.
KW - Ca-signaling
KW - FOR20
KW - Interactome analysis
KW - Protein array
KW - S100A6
UR - http://www.scopus.com/inward/record.url?scp=85026867249&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026867249&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2017.07.161
DO - 10.1016/j.bbrc.2017.07.161
M3 - Article
C2 - 28765046
AN - SCOPUS:85026867249
VL - 491
SP - 980
EP - 985
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -