Hypoxia promotes progesterone synthesis during luteinization in bovine granulosa cells

[No Value] Fadhillah, Shin Yoshioka, Ryo Nishimura, Kiyoshi Okuda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To determine whether hypoxia has an effect on luteinization, we examined the influence of hypoxia on a model of bovine luteinizing and non-luteinizing granulosa cell culture. The granulosa cells were obtained from small antral follicles (≤ 6 mm in diameter). To induce luteinization, the cells were treated for 24 h with insulin (2 μg/ml), forskolin (10 μM) or insulin in combination with forskolin at 20% O2. After 24 h, progesterone (P4) production was higher in the treated cells, which we defined as luteinizing granulosa cells, than in non-treated cells, which we defined as non-luteinizing granulosa cells. P4 production by non-luteinizing granulosa cells was not affected by hypoxia (24 h at 10% and 5% O2), while P4 production by granulosa cells treated with insulin in combination with forskolin was significantly increased under hypoxia (24 h at 10% and 5% O2). Because hypoxia affected P4 production by the luteinizing granulosa cells but not by the non-luteinizing granulosa cells, hypoxia seems to promote P4 production during, rather than before, luteinization. In the cells treated with insulin in combination with forskolin, mRNA and protein expression of steroidogenic acute regulatory protein (StAR) and protein expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) increased under 10% O2, while mRNA and protein expressions of key protein and enzymes in P4 biosynthesis did not increase under 5% O2. The overall results suggest that hypoxia plays a role in progressing and completing the luteinization by enhancing P4 production through StAR as well as 3β-HSD expressions in the early time of establishing the corpus luteum.

Original languageEnglish
Pages (from-to)194-201
Number of pages8
JournalJournal of Reproduction and Development
Volume60
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Luteinization
luteinization
Granulosa Cells
granulosa cells
Progesterone
hypoxia
progesterone
forskolin
synthesis
cattle
Colforsin
insulin
3-Hydroxysteroid Dehydrogenases
Insulin
hydroxysteroid dehydrogenases
protein synthesis
regulatory proteins
Proteins
cells
Cell Hypoxia

Keywords

  • Granulosa cells
  • Hypoxia
  • Luteinization
  • Progesterone

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Medicine(all)

Cite this

Hypoxia promotes progesterone synthesis during luteinization in bovine granulosa cells. / Fadhillah, [No Value]; Yoshioka, Shin; Nishimura, Ryo; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 60, No. 3, 2014, p. 194-201.

Research output: Contribution to journalArticle

Fadhillah, [No Value] ; Yoshioka, Shin ; Nishimura, Ryo ; Okuda, Kiyoshi. / Hypoxia promotes progesterone synthesis during luteinization in bovine granulosa cells. In: Journal of Reproduction and Development. 2014 ; Vol. 60, No. 3. pp. 194-201.
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