Hypoxia promotes luteal cell death in bovine corpus luteum

Ryo Nishimura, Junichi Komiyama, Yukari Tasaki, Tomas J. Acosta, Kiyoshi Okuda

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Low oxygen caused by a decreasing blood supply is known to induce various responses of cells, including apoptosis. The present study was conducted to examine whether low-oxygen conditions (hypoxia) induce luteal cell apoptosis in cattle. Bovine midluteal cells incubated under hypoxia (3% O2) showed significantly more cell death than did those incubated under normoxia (20% O2) at 24 and 48 h of culture, and had significantly lower progesterone (P4) levels starting at 8 h. Characteristic features of apoptosis, such as shrunken nuclei and DNA fragmentation, were observed in cells cultured under hypoxia for 48 h. Hypoxia increased the mRNA expressions of BNIP3 and caspase 3 at 24 and 48 h of culture. Hypoxia had no significant effect on the expressions of BCL2 and BAX mRNA. Hypoxia also increased BNIP3 protein, and activated capsase-3. Treatment of P4 attenuated cell death, caspase-3 mRNA expression, and caspase-3 activity under hypoxia. Overall results of the present study indicate that hypoxia induces luteal cell apoptosis by enhancing the expression of proapoptotic protein, BNIP3, and by activating caspase-3, and that the induction of apoptosis by hypoxia is partially caused by a decrease in P4 production. Because hypoxia suppresses P4 synthesis in bovine luteal cells, we suggest that oxygen deficiency caused by a decreasing blood supply in bovine corpus luteum is one of the major factors contributing to both functional and structural luteolysis.

Original languageEnglish
Pages (from-to)529-536
Number of pages8
JournalBiology of reproduction
Volume78
Issue number3
DOIs
Publication statusPublished - Mar 1 2008

Keywords

  • Apoptosis
  • Corpus luteum
  • Hypoxia
  • Progesterone
  • Signal transduction

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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  • Cite this

    Nishimura, R., Komiyama, J., Tasaki, Y., Acosta, T. J., & Okuda, K. (2008). Hypoxia promotes luteal cell death in bovine corpus luteum. Biology of reproduction, 78(3), 529-536. https://doi.org/10.1095/biolreprod.107.063370