Hypoxia is important for establishing vascularization during corpus luteum formation in cattle

Ryo Nishimura, Kiyoshi Okuda

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Hypoxia-inducible factor 1 (HIF1) has been demonstrated to have critical roles in angiogenesis via transcriptional regulation of angiogenic factors, such as vascular endothelial growth factor (VEGF). In the ovary, angiogenesis is known to occur after ovulation in the developing corpus luteum (CL) in mammals. To determine whether HIF1 participates in angiogenesis in bovine CL, the present study investigated the mRNA and protein expressions of the HIF1 alpha subunit (HIF1A) and VEGF in bovine CL during the estrous cycle. The effects of hypoxia on the expressions of HIF1A protein, VEGF mRNA and VEGF protein in bovine luteal cells were also examined by using a cell culture system. HIF1A mRNA expression was less at the regressed stage than at the other stages, whereas protein expression of HIF1A was highest at the early luteal stage and decreased thereafter. VEGF mRNA expression was highest at the developing luteal stage and decreased thereafter. VEGF protein expression was highest at the early luteal stage and decreased significantly at the regressed luteal stage. Hypoxia increased the amounts of HIF1A protein, VEGF mRNA and VEGF protein in cultured bovine luteal cells. Furthermore, we found that hypoxia inhibited progesterone production in the mid luteal cells, but not in the early luteal cells. The overall findings indicate that HIF1 is one of the factors promoting VEGF-induced angiogenesis during luteal development, and suggest that the hypoxic conditions formed after follicle rupture contribute to establishing luteal vascularization in cattle.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalJournal of Reproduction and Development
Volume56
Issue number1
DOIs
Publication statusPublished - Feb 2010

Fingerprint

vascular endothelial growth factors
corpus luteum
hypoxia
luteal cells
cattle
angiogenesis
protein synthesis
protein subunits
anaerobic conditions
estrous cycle
ovulation
progesterone
cell culture
proteins
mammals
hypoxia-inducible factor 1

Keywords

  • Cattle
  • Corpus luteum
  • Hypoxia
  • Hypoxia-inducible factor 1
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Hypoxia is important for establishing vascularization during corpus luteum formation in cattle. / Nishimura, Ryo; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 56, No. 1, 02.2010, p. 110-116.

Research output: Contribution to journalArticle

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