Hypoxia increases transforming growth factor-β1 concomitantly with types I and III collagen without further enhancement by reoxygenation in cultured rat cardiac fibroblasts

H. Nunoyama, S. Kusachi, Y. Ninomiya, Toshitaka Oohashi, J. Kondo, M. Murakami, T. Murakami, H. Ohnishi, M. Doi, T. Tsuji

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effects of oxygen concentration and reoxygenation following hypoxic culture on transforming growth factor-β1 (TGF-β1) expression and types I and III collagen [α2(I) and α1(III)] mRNA expression were examined in neonatal rat cardiac fibroblasts cultured with medium containing 10% or 1% fetal calf serum at confluency. The oxygen tension was varied using a N2- CO2-O2 controllable incubator. Cells were exposed to continuous hypoxia (2% O2) or were reoxygenated (21% O2) after hypoxic culture. The TGF-β1 protein expression level was determined by an enzyme-linked immunosorbent assay, and the expression levels of TGF-β1, α2(I) and α1(III) mRNA were measured by a Northern blot analysis. Both the TGF-β1 mRNA and protein levels were higher in the continuous hypoxic cultures than in the continuous normoxic cultures. Their levels in reoxygenation cultures were not substantially different from those in the continuous normoxic cultures. Similarly, the expressions of both α2(I) and α1(III) mRNA were enhanced in continuous hypoxic culture, and no differences were obtained between normoxic and reoxygenation cultures. The present results indicate that hypoxia induces high expression levels of TGF-β1 and types I and III collagen in rat cardiac fibroblasts and that reoxygenation does not further enhance this hypoxia- induced upregulation.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalConnective Tissue
Volume30
Issue number3
Publication statusPublished - 1998

Fingerprint

Collagen Type III
Transforming Growth Factors
Collagen Type I
Fibroblasts
Messenger RNA
Oxygen
Incubators
Northern Blotting
Proteins
Up-Regulation
Enzyme-Linked Immunosorbent Assay
Hypoxia
Serum

Keywords

  • Cytokine
  • Enzyme linked immunoassay
  • Extracellular matrix
  • Growth factor
  • Northern blot analysis
  • Reperfusion

ASJC Scopus subject areas

  • Rheumatology

Cite this

Hypoxia increases transforming growth factor-β1 concomitantly with types I and III collagen without further enhancement by reoxygenation in cultured rat cardiac fibroblasts. / Nunoyama, H.; Kusachi, S.; Ninomiya, Y.; Oohashi, Toshitaka; Kondo, J.; Murakami, M.; Murakami, T.; Ohnishi, H.; Doi, M.; Tsuji, T.

In: Connective Tissue, Vol. 30, No. 3, 1998, p. 207-211.

Research output: Contribution to journalArticle

Nunoyama, H, Kusachi, S, Ninomiya, Y, Oohashi, T, Kondo, J, Murakami, M, Murakami, T, Ohnishi, H, Doi, M & Tsuji, T 1998, 'Hypoxia increases transforming growth factor-β1 concomitantly with types I and III collagen without further enhancement by reoxygenation in cultured rat cardiac fibroblasts', Connective Tissue, vol. 30, no. 3, pp. 207-211.
Nunoyama, H. ; Kusachi, S. ; Ninomiya, Y. ; Oohashi, Toshitaka ; Kondo, J. ; Murakami, M. ; Murakami, T. ; Ohnishi, H. ; Doi, M. ; Tsuji, T. / Hypoxia increases transforming growth factor-β1 concomitantly with types I and III collagen without further enhancement by reoxygenation in cultured rat cardiac fibroblasts. In: Connective Tissue. 1998 ; Vol. 30, No. 3. pp. 207-211.
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