Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage

Ryo Nishimura, Hiroki Hasegawa, Masamichi Yamashita, Norihiko Ito, Yoshiharu Okamoto, Takashi Takeuchi, Tomoaki Kubo, Kosuke Iga, Koji Kimura, Mitsugu Hishinuma, Kiyoshi Okuda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells. GLUT1 mRNA expression in bovine CL was higher at the early luteal stage compared to the other later stages. Hypoxia (3% O2) increased GLUT1 mRNA expression in early luteal cells, but not in mid luteal cells. Glucose (0-25 mM) increased P4 production in early luteal cells, but not in mid luteal cells. Both GLUT1 inhibitors decreased P4 production in early and mid luteal cells. Overall, the results suggest that GLUT1 (possibly induced by hypoxic conditions in the early CL) plays a role in the establishment and development of bovine CL, especially in supporting luteal P4 synthesis at the early luteal stage.

Original languageEnglish
Pages (from-to)1878-1883
Number of pages6
JournalJournal of Veterinary Medical Science
Volume79
Issue number11
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

glucose transporters
Facilitative Glucose Transport Proteins
Corpus Luteum
corpus luteum
hypoxia
Luteal Cells
luteal cells
cattle
anaerobic conditions
Messenger RNA
Hypoxia
Glucose
Hypoxia-Inducible Factor 1
cytochalasin B
glucose
Cytochalasin B
Ovulation
Progesterone
ovulation
progesterone

Keywords

  • Corpus luteum
  • Glucose transporter-1
  • HIF1
  • Hypoxia
  • Progesterone

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Nishimura, R., Hasegawa, H., Yamashita, M., Ito, N., Okamoto, Y., Takeuchi, T., ... Okuda, K. (2017). Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage. Journal of Veterinary Medical Science, 79(11), 1878-1883. https://doi.org/10.1292/jvms.17-0284

Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage. / Nishimura, Ryo; Hasegawa, Hiroki; Yamashita, Masamichi; Ito, Norihiko; Okamoto, Yoshiharu; Takeuchi, Takashi; Kubo, Tomoaki; Iga, Kosuke; Kimura, Koji; Hishinuma, Mitsugu; Okuda, Kiyoshi.

In: Journal of Veterinary Medical Science, Vol. 79, No. 11, 01.11.2017, p. 1878-1883.

Research output: Contribution to journalArticle

Nishimura, R, Hasegawa, H, Yamashita, M, Ito, N, Okamoto, Y, Takeuchi, T, Kubo, T, Iga, K, Kimura, K, Hishinuma, M & Okuda, K 2017, 'Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage', Journal of Veterinary Medical Science, vol. 79, no. 11, pp. 1878-1883. https://doi.org/10.1292/jvms.17-0284
Nishimura, Ryo ; Hasegawa, Hiroki ; Yamashita, Masamichi ; Ito, Norihiko ; Okamoto, Yoshiharu ; Takeuchi, Takashi ; Kubo, Tomoaki ; Iga, Kosuke ; Kimura, Koji ; Hishinuma, Mitsugu ; Okuda, Kiyoshi. / Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage. In: Journal of Veterinary Medical Science. 2017 ; Vol. 79, No. 11. pp. 1878-1883.
@article{114b6f35b24845a4a1c41f91b4846659,
title = "Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage",
abstract = "A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells. GLUT1 mRNA expression in bovine CL was higher at the early luteal stage compared to the other later stages. Hypoxia (3{\%} O2) increased GLUT1 mRNA expression in early luteal cells, but not in mid luteal cells. Glucose (0-25 mM) increased P4 production in early luteal cells, but not in mid luteal cells. Both GLUT1 inhibitors decreased P4 production in early and mid luteal cells. Overall, the results suggest that GLUT1 (possibly induced by hypoxic conditions in the early CL) plays a role in the establishment and development of bovine CL, especially in supporting luteal P4 synthesis at the early luteal stage.",
keywords = "Corpus luteum, Glucose transporter-1, HIF1, Hypoxia, Progesterone",
author = "Ryo Nishimura and Hiroki Hasegawa and Masamichi Yamashita and Norihiko Ito and Yoshiharu Okamoto and Takashi Takeuchi and Tomoaki Kubo and Kosuke Iga and Koji Kimura and Mitsugu Hishinuma and Kiyoshi Okuda",
year = "2017",
month = "11",
day = "1",
doi = "10.1292/jvms.17-0284",
language = "English",
volume = "79",
pages = "1878--1883",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "11",

}

TY - JOUR

T1 - Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage

AU - Nishimura, Ryo

AU - Hasegawa, Hiroki

AU - Yamashita, Masamichi

AU - Ito, Norihiko

AU - Okamoto, Yoshiharu

AU - Takeuchi, Takashi

AU - Kubo, Tomoaki

AU - Iga, Kosuke

AU - Kimura, Koji

AU - Hishinuma, Mitsugu

AU - Okuda, Kiyoshi

PY - 2017/11/1

Y1 - 2017/11/1

N2 - A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells. GLUT1 mRNA expression in bovine CL was higher at the early luteal stage compared to the other later stages. Hypoxia (3% O2) increased GLUT1 mRNA expression in early luteal cells, but not in mid luteal cells. Glucose (0-25 mM) increased P4 production in early luteal cells, but not in mid luteal cells. Both GLUT1 inhibitors decreased P4 production in early and mid luteal cells. Overall, the results suggest that GLUT1 (possibly induced by hypoxic conditions in the early CL) plays a role in the establishment and development of bovine CL, especially in supporting luteal P4 synthesis at the early luteal stage.

AB - A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells. GLUT1 mRNA expression in bovine CL was higher at the early luteal stage compared to the other later stages. Hypoxia (3% O2) increased GLUT1 mRNA expression in early luteal cells, but not in mid luteal cells. Glucose (0-25 mM) increased P4 production in early luteal cells, but not in mid luteal cells. Both GLUT1 inhibitors decreased P4 production in early and mid luteal cells. Overall, the results suggest that GLUT1 (possibly induced by hypoxic conditions in the early CL) plays a role in the establishment and development of bovine CL, especially in supporting luteal P4 synthesis at the early luteal stage.

KW - Corpus luteum

KW - Glucose transporter-1

KW - HIF1

KW - Hypoxia

KW - Progesterone

UR - http://www.scopus.com/inward/record.url?scp=85034633654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034633654&partnerID=8YFLogxK

U2 - 10.1292/jvms.17-0284

DO - 10.1292/jvms.17-0284

M3 - Article

C2 - 29046497

AN - SCOPUS:85034633654

VL - 79

SP - 1878

EP - 1883

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 11

ER -