Hypotonic buffer induces meiosis and formation of anucleate cytoplasmic islands in the egg of the two-spotted cricket Gryllus bimaculatus

Isao Sarashina, Yohei Shinmyo, Ayumi Hirose, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi, Tetsuya Horio, Sumihare Noji

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In insects, egg activation is known to occur in vivo and independently of fertilization, but its mechanisms are poorly understood. To gain understanding of these mechanisms, an attempt was made to activate the egg of Gryllus bimaculatus in vitro. It was found that meiosis resumed and was completed in unfertilized eggs treated with hypotonic buffer. Early developmental processes in activated, unfertilized eggs were investigated and compared with those in fertilized eggs. Mitosis did not progress, resulting in formation of anucleate cytoplasmic islands (pseudoenergids). Development in the activated, unfertilized eggs stopped at this stage and both yolk subdivision and cellularization did not occur. To elucidate the role of the nucleus in the developmental process to the syncytial stage in fertilized eggs, eggs were treated with aphidicolin to inhibit DNA polymerization. It was found that pseudoenergids also formed in these aphidicolin-treated fertilized eggs. These results demonstrate that pseudoenergids can increase in number independently of nuclei, suggesting that the cytoplasm rather than the nucleus plays the primary role in development to the syncytial stage in G. bimaculatus.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalDevelopment Growth and Differentiation
Volume45
Issue number2
DOIs
Publication statusPublished - Apr 2003
Externally publishedYes

Fingerprint

Gryllidae
Meiosis
Islands
Ovum
Buffers
Zygote
Aphidicolin
Mitosis
Fertilization
Polymerization
Eggs
Insects
Cytoplasm
DNA

Keywords

  • Aphidicolin
  • Egg activation
  • Energid
  • Gryllus bimaculatus
  • Hypotonic buffer

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Hypotonic buffer induces meiosis and formation of anucleate cytoplasmic islands in the egg of the two-spotted cricket Gryllus bimaculatus. / Sarashina, Isao; Shinmyo, Yohei; Hirose, Ayumi; Miyawaki, Katsuyuki; Mito, Taro; Ohuchi, Hideyo; Horio, Tetsuya; Noji, Sumihare.

In: Development Growth and Differentiation, Vol. 45, No. 2, 04.2003, p. 103-112.

Research output: Contribution to journalArticle

Sarashina, Isao ; Shinmyo, Yohei ; Hirose, Ayumi ; Miyawaki, Katsuyuki ; Mito, Taro ; Ohuchi, Hideyo ; Horio, Tetsuya ; Noji, Sumihare. / Hypotonic buffer induces meiosis and formation of anucleate cytoplasmic islands in the egg of the two-spotted cricket Gryllus bimaculatus. In: Development Growth and Differentiation. 2003 ; Vol. 45, No. 2. pp. 103-112.
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