Hypersensitivity of a Urate-null Strain of Drosophila melanogaster to the Toxic Effects of Environmental Cigarette Smoke

Yuko Hamatake, Ayumi Morita, Yoko Yuma, Keinosuke Okamoto, Sakae Arimoto, Toshinori Suzuki, Hiroshi Kasai, Kazuaki Kawai, Tomoe Negishi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Epidemiological evidence indicates that cigarette smoke is harmful to human health. Mainstream cigarette smoke has already been demonstrated to induce tissue and cellular damage in animal models. In the present study, we examined the toxicity of environmental cigarette smoke (ECS) by exposing Drosophila melanogaster larvae from urate-null and wild-type strains to ECS for 3 or 6 h at the third instar stage. We then determined survival to adulthood and the fecundity of adult females that survived larval ECS exposure. The survival of the urate-null strain, but not the wild-type strain, decreased significantly in an exposure-dependent manner. Moreover, the fecundity of treated urate-null, but not wild-type, females decreased significantly relative to the control level, irrespective of mating partner exposure to ECS at the larvae stage. These results demonstrate the killing effect and reproductive toxicity of ECS on urate-null larvae of Drosophila. Since the urate-null strain is known to be sensitive to oxidative agents, we propose that the main cause of the observed toxic effects of ECS is oxidative stress. Key words: environmental cigarette smoke, urate-null strain, survival, Drosophila, fecundity.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalGenes and Environment
Volume31
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Poisons
Uric Acid
Drosophila melanogaster
Smoke
Tobacco Products
smoke
Hypersensitivity
Fertility
Larva
fecundity
larva
Drosophila
Survival
toxicity
toxic effect
Environmental Exposure
Oxidative Stress
Animal Models
damage
animal

ASJC Scopus subject areas

  • Social Psychology
  • Genetics
  • Environmental Science (miscellaneous)

Cite this

Hypersensitivity of a Urate-null Strain of Drosophila melanogaster to the Toxic Effects of Environmental Cigarette Smoke. / Hamatake, Yuko; Morita, Ayumi; Yuma, Yoko; Okamoto, Keinosuke; Arimoto, Sakae; Suzuki, Toshinori; Kasai, Hiroshi; Kawai, Kazuaki; Negishi, Tomoe.

In: Genes and Environment, Vol. 31, No. 2, 2009, p. 43-46.

Research output: Contribution to journalArticle

Hamatake, Yuko ; Morita, Ayumi ; Yuma, Yoko ; Okamoto, Keinosuke ; Arimoto, Sakae ; Suzuki, Toshinori ; Kasai, Hiroshi ; Kawai, Kazuaki ; Negishi, Tomoe. / Hypersensitivity of a Urate-null Strain of Drosophila melanogaster to the Toxic Effects of Environmental Cigarette Smoke. In: Genes and Environment. 2009 ; Vol. 31, No. 2. pp. 43-46.
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