TY - JOUR
T1 - Hyperpolarization-activated cyclic nucleotide gated channels
T2 - A potential molecular link between epileptic seizures and Aβ generation in Alzheimer's disease
AU - Saito, Yuhki
AU - Inoue, Tsuyoshi
AU - Zhu, Gang
AU - Kimura, Naoki
AU - Okada, Motohiro
AU - Nishimura, Masaki
AU - Kimura, Nobuyuki
AU - Murayama, Shigeo
AU - Kaneko, Sunao
AU - Shigemoto, Ryuichi
AU - Imoto, Keiji
AU - Suzuki, Toshiharu
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Research Activity Start-up (21890002) and by a Grant-in-Aid for Young Scientists (B) (23790069) from the Japan Society for the Promotion of Science (JSPS) to YS. YS was also supported by the Akiyama Life Science Foundation and the Regional R&D Proposal-Based Program from the Northern Advancement Center for Science & Technology of Hokkaido, Japan. TS was supported in part by Grants-in-aid for Scientific Research (2339001, 2311370, 22659011) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and by a grant from the Ministry of Health, Labor and Welfare (MHLW) of Japan.
PY - 2012
Y1 - 2012
N2 - Background: One of the best-characterized causative factors of Alzheimer's disease (AD) is the generation of amyloid-β peptide (Aβ). AD subjects are at high risk of epileptic seizures accompanied by aberrant neuronal excitability, which in itself enhances Aβ generation. However, the molecular linkage between epileptic seizures and Aβ generation in AD remains unclear. Results: X11 and X11-like (X11L) gene knockout mice suffered from epileptic seizures, along with a malfunction of hyperpolarization-activated cyclic nucleotide gated (HCN) channels. Genetic ablation of HCN1 in mice and HCN1 channel blockage in cultured Neuro2a (N2a) cells enhanced Aβ generation. Interestingly, HCN1 levels dramatically decreased in the temporal lobe of cynomolgus monkeys (Macaca fascicularis) during aging and were significantly diminished in the temporal lobe of sporadic AD patients. Conclusion: Because HCN1 associates with amyloid-β precursor protein (APP) and X11/X11L in the brain, genetic deficiency of X11/X11L may induce aberrant HCN1 distribution along with epilepsy. Moreover, the reduction in HCN1 levels in aged primates may contribute to augmented Aβ generation. Taken together, HCN1 is proposed to play an important role in the molecular linkage between epileptic seizures and Aβ generation, and in the aggravation of sporadic AD.
AB - Background: One of the best-characterized causative factors of Alzheimer's disease (AD) is the generation of amyloid-β peptide (Aβ). AD subjects are at high risk of epileptic seizures accompanied by aberrant neuronal excitability, which in itself enhances Aβ generation. However, the molecular linkage between epileptic seizures and Aβ generation in AD remains unclear. Results: X11 and X11-like (X11L) gene knockout mice suffered from epileptic seizures, along with a malfunction of hyperpolarization-activated cyclic nucleotide gated (HCN) channels. Genetic ablation of HCN1 in mice and HCN1 channel blockage in cultured Neuro2a (N2a) cells enhanced Aβ generation. Interestingly, HCN1 levels dramatically decreased in the temporal lobe of cynomolgus monkeys (Macaca fascicularis) during aging and were significantly diminished in the temporal lobe of sporadic AD patients. Conclusion: Because HCN1 associates with amyloid-β precursor protein (APP) and X11/X11L in the brain, genetic deficiency of X11/X11L may induce aberrant HCN1 distribution along with epilepsy. Moreover, the reduction in HCN1 levels in aged primates may contribute to augmented Aβ generation. Taken together, HCN1 is proposed to play an important role in the molecular linkage between epileptic seizures and Aβ generation, and in the aggravation of sporadic AD.
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U2 - 10.1186/1750-1326-7-50
DO - 10.1186/1750-1326-7-50
M3 - Article
C2 - 23034178
AN - SCOPUS:84866873047
VL - 7
JO - Molecular Neurodegeneration
JF - Molecular Neurodegeneration
SN - 1750-1326
IS - 1
M1 - 50
ER -