Hyperosmotic tolerance of adult fish and early embryos are determined by discrete, single loci in the genus Oryzias

Taijun Myosho, Hideya Takahashi, Kento Yoshida, Tadashi Sato, Satoshi Hamaguchi, Tatsuya Sakamoto, Mitsuru Sakaizumi

Research output: Contribution to journalArticle

Abstract

The acquisition of environmental osmolality tolerance traits in individuals and gametes is an important event in the evolution and diversification of organisms. Although teleost fish exhibit considerable intra- and interspecific variation in salinity tolerance, the genetic mechanisms underlying this trait remain unclear. Oryzias celebensis survives in sea and fresh water during both the embryonic and adult stages, whereas its close relative Oryzias woworae cannot survive in sea water at either stage. A linkage analysis using backcross progeny identified a single locus responsible for adult hyperosmotic tolerance on a fused chromosome that corresponds to O. latipes linkage groups (LGs) 6 and 23. Conversely, O. woworae eggs fertilised with O. celebensis sperm died in sea water at the cleavage stages, whereas O. celebensis eggs fertilised with O. woworae sperm developed normally, demonstrating that maternal factor(s) from O. celebensis are responsible for hyperosmotic tolerance during early development. A further linkage analysis using backcrossed females revealed a discrete single locus relating to the maternal hyperosmotic tolerance factor in a fused chromosomal region homologous to O. latipes LGs 17 and 19. These results indicate that a maternal factor governs embryonic hyperosmotic tolerance and maps to a locus distinct from that associated with adult hyperosmotic tolerance.

Original languageEnglish
Article number6897
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Oryzias
Leptothrium
embryo (animal)
seawater
linkage groups
loci
linkage (genetics)
fish
spermatozoa
osmolality
interspecific variation
early development
germ cells
salinity
chromosomes
organisms

ASJC Scopus subject areas

  • General

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Hyperosmotic tolerance of adult fish and early embryos are determined by discrete, single loci in the genus Oryzias. / Myosho, Taijun; Takahashi, Hideya; Yoshida, Kento; Sato, Tadashi; Hamaguchi, Satoshi; Sakamoto, Tatsuya; Sakaizumi, Mitsuru.

In: Scientific Reports, Vol. 8, No. 1, 6897, 01.12.2018.

Research output: Contribution to journalArticle

Myosho, Taijun ; Takahashi, Hideya ; Yoshida, Kento ; Sato, Tadashi ; Hamaguchi, Satoshi ; Sakamoto, Tatsuya ; Sakaizumi, Mitsuru. / Hyperosmotic tolerance of adult fish and early embryos are determined by discrete, single loci in the genus Oryzias. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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