Hyperlipidemia is involved in apoptosis in rat submandibular glands

Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Toshiki Yoneda, Yoshihiro Shimazaki, Manabu Morita

Research output: Contribution to journalArticle

Abstract

Objective The aim of the present study was to investigate the effects of hyperlipidemia on histological changes and apoptosis in submandibular glands using apolipoprotein E (apoE)-deficient rats. Design Histopathological findings related to induced apoptosis in the submandibular glands were compared between apoE-deficient rats (n = 6; male; age, 16 weeks) and the corresponding wild-type rats (n = 6). Results ApoE-deficient rats showed significantly higher plasma levels of oxidized low-density lipoprotein (LDL), total cholesterol, very LDL and LDL, and lower plasma levels of high-density lipoprotein when compared to control rats (P < 0.05). Lipid deposition in the submandibular gland was observed in apoE-deficient rat group and in none of the control group. Significant increases in vacuolization and apoptosis in acinar cells were observed in apoE-deficient rats, as compared to control rats (P < 0.05). The number of active caspase-3-positive cells was also higher in the apoE-deficient rat group when compared with the control group (P < 0.01). Conclusions According to our results, hyperlipidemia induced apoptosis in apoE-deficient rat submandibular glands. Oxidized LDL generation in case of hyperlipidemia may trigger off a reaction of apoptotic acinar cells with vacuolization in the submandibular glands.

Original languageEnglish
Pages (from-to)136-140
Number of pages5
JournalArchives of Oral Biology
Volume81
DOIs
Publication statusPublished - Sep 1 2017

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Submandibular Gland
Hyperlipidemias
Apolipoproteins E
Apoptosis
Acinar Cells
Control Groups
VLDL Lipoproteins
HDL Lipoproteins
LDL Lipoproteins
Caspase 3
LDL Cholesterol
Lipids

Keywords

  • Apoptosis
  • Hyperlipidemia
  • Rats
  • Submandibular gland

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Hyperlipidemia is involved in apoptosis in rat submandibular glands. / Irie, Koichiro; Tomofuji, Takaaki; Ekuni, Daisuke; Azuma, Tetsuji; Yoneda, Toshiki; Shimazaki, Yoshihiro; Morita, Manabu.

In: Archives of Oral Biology, Vol. 81, 01.09.2017, p. 136-140.

Research output: Contribution to journalArticle

Irie, Koichiro ; Tomofuji, Takaaki ; Ekuni, Daisuke ; Azuma, Tetsuji ; Yoneda, Toshiki ; Shimazaki, Yoshihiro ; Morita, Manabu. / Hyperlipidemia is involved in apoptosis in rat submandibular glands. In: Archives of Oral Biology. 2017 ; Vol. 81. pp. 136-140.
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