Abstract
Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.
| Original language | English |
|---|---|
| Pages (from-to) | 601-605 |
| Number of pages | 5 |
| Journal | Archives of Virology |
| Volume | 155 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2010 |
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ASJC Scopus subject areas
- Virology
Cite this
Hydroxyurea as an inhibitor of hepatitis C virus RNA replication. / Nozaki, Akito; Morimoto, Manabu; Kondo, Masaaki; Oshima, Takashi; Numata, Kazushi; Fujisawa, Shin; Kaneko, Takeshi; Miyajima, Eiji; Morita, Satoshi; Mori, Kyoko; Ikeda, Masanori; Kato, Nobuyuki; Tanaka, Katsuaki.
In: Archives of Virology, Vol. 155, No. 4, 04.2010, p. 601-605.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hydroxyurea as an inhibitor of hepatitis C virus RNA replication
AU - Nozaki, Akito
AU - Morimoto, Manabu
AU - Kondo, Masaaki
AU - Oshima, Takashi
AU - Numata, Kazushi
AU - Fujisawa, Shin
AU - Kaneko, Takeshi
AU - Miyajima, Eiji
AU - Morita, Satoshi
AU - Mori, Kyoko
AU - Ikeda, Masanori
AU - Kato, Nobuyuki
AU - Tanaka, Katsuaki
PY - 2010/4
Y1 - 2010/4
N2 - Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.
AB - Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.
UR - http://www.scopus.com/inward/record.url?scp=77950601184&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950601184&partnerID=8YFLogxK
U2 - 10.1007/s00705-010-0624-1
DO - 10.1007/s00705-010-0624-1
M3 - Article
C2 - 20204428
AN - SCOPUS:77950601184
VL - 155
SP - 601
EP - 605
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 4
ER -