Hydroxyurea as an inhibitor of hepatitis C virus RNA replication

Akito Nozaki; Manabu Morimoto; Masaaki Kondo; Takashi Oshima; Kazushi Numata; Shin Fujisawa; Takeshi Kaneko; Eiji Miyajima; Satoshi Morita; Kyoko Mori; Masanori Ikeda; Nobuyuki Kato; Katsuaki Tanaka

doi:10.1007/s00705-010-0624-1

Hydroxyurea as an inhibitor of hepatitis C virus RNA replication

Akito Nozaki, Manabu Morimoto, Masaaki Kondo, Takashi Oshima, Kazushi Numata, Shin Fujisawa, Takeshi Kaneko, Eiji Miyajima, Satoshi Morita, Kyoko Mori, Masanori Ikeda, Nobuyuki Kato, Katsuaki Tanaka

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.

Original language	English
Pages (from-to)	601-605
Number of pages	5
Journal	Archives of Virology
Volume	155
Issue number	4
DOIs	https://doi.org/10.1007/s00705-010-0624-1
Publication status	Published - Apr 2010
Externally published	Yes

ASJC Scopus subject areas

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00705-010-0624-1

Cite this

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title = "Hydroxyurea as an inhibitor of hepatitis C virus RNA replication",

abstract = "Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.",

author = "Akito Nozaki and Manabu Morimoto and Masaaki Kondo and Takashi Oshima and Kazushi Numata and Shin Fujisawa and Takeshi Kaneko and Eiji Miyajima and Satoshi Morita and Kyoko Mori and Masanori Ikeda and Nobuyuki Kato and Katsuaki Tanaka",

note = "Funding Information: The authors thank Kazue Yoshihara and Yoshiko Kubushiro for their technical assistance. This work was supported by a grant for the 2008 Strategic Research Project (No. W20012) provided by Yokohama City University, Japan.",

year = "2010",

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doi = "10.1007/s00705-010-0624-1",

language = "English",

volume = "155",

pages = "601--605",

journal = "Archives of Virology",

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T1 - Hydroxyurea as an inhibitor of hepatitis C virus RNA replication

AU - Nozaki, Akito

AU - Morimoto, Manabu

AU - Kondo, Masaaki

AU - Oshima, Takashi

AU - Numata, Kazushi

AU - Fujisawa, Shin

AU - Kaneko, Takeshi

AU - Miyajima, Eiji

AU - Morita, Satoshi

AU - Mori, Kyoko

AU - Ikeda, Masanori

AU - Kato, Nobuyuki

AU - Tanaka, Katsuaki

N1 - Funding Information: The authors thank Kazue Yoshihara and Yoshiko Kubushiro for their technical assistance. This work was supported by a grant for the 2008 Strategic Research Project (No. W20012) provided by Yokohama City University, Japan.

PY - 2010/4

Y1 - 2010/4

N2 - Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.

AB - Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.

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JO - Archives of Virology

JF - Archives of Virology

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ER -

Hydroxyurea as an inhibitor of hepatitis C virus RNA replication

Abstract

ASJC Scopus subject areas

UN SDGs

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