Zn-substituted apatites with homogeneous Zn distribution in the particles (ZnΦHAp) and those with graded distribution (Zn/HAp) were prepared via a conventional wet chemical synthesis and soaking the Zn-free apatite particles in zinc nitrate solutions: X-ray photoelectron spectra of the apatites confirmed such Zn distribution. Physical and chemical properties such as particle size, specific surface area, or zeta-potential and were correlated to adsorption of β2-microglobulin (β2-MG) and bovine serum albumin (BSA). Adsorption of β2-MG on both apatites increased with the Zn content, whereas more β2-MG was adsorbed on Zn/HAp than on ZnΦHAp. The amount of BSA adsorbed on ZnΦHAp reached a maximum at about 1.5%, while it remained constant for Zn/HAp. From the results, it was suggested that Zn-substituted sites had better affinity against β2-MG and favored β2-MG adsorption. Surface charge on the particles was one of the factors to explain the suppressed adsorption of BSA by both apatites.