Abstract
Hydroxyapatite (HAp), the major inorganic component of hard tissues in vivo, was formed in/on a PVA gel. The HAp formation ratio depended on the reaction cycle number, but was independent of the alternate soaking period per cycle. The Ca/P molar ratio of HAp formed at 10 reaction cycles was very close to the theoretical value of HAp, 1.67. CHO-K1 cell adhesion, proliferation and maximum cell density on HAp plates were better on plates formed at two or five reaction cycles using 200 mM CaCl2 and 120 mM Na2HPO 4 solutions than on plates formed under other conditions. Furthermore, the adhesion ratio of CHO-K1 cells on HAp plates formed at 10 reaction cycles was about 60% of those at two or five reaction cycles.
| Original language | English |
|---|---|
| Pages (from-to) | 155-161 |
| Number of pages | 7 |
| Journal | Biochemical Engineering Journal |
| Volume | 20 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Aug 15 2004 |
| Externally published | Yes |
Keywords
- Alternate soaking process
- Animal cell culture
- Biomedical
- Culture substratum
- Hydroxyapatite
- Optimization
ASJC Scopus subject areas
- Biotechnology
- Environmental Engineering
- Bioengineering
- Biomedical Engineering
Fingerprint Dive into the research topics of 'Hydroxyapatite for use as an animal cell culture substratum obtained by an alternate soaking process'. Together they form a unique fingerprint.
Cite this
Ijima, H., Ohchi, T., Ono, T., & Kawakami, K. (2004). Hydroxyapatite for use as an animal cell culture substratum obtained by an alternate soaking process. Biochemical Engineering Journal, 20(2-3), 155-161. https://doi.org/10.1016/j.bej.2003.07.004