Hydroxyapatite for use as an animal cell culture substratum obtained by an alternate soaking process

Hiroyuki Ijima, Tomokazu Ohchi, Tsutomu Ono, Koei Kawakami

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hydroxyapatite (HAp), the major inorganic component of hard tissues in vivo, was formed in/on a PVA gel. The HAp formation ratio depended on the reaction cycle number, but was independent of the alternate soaking period per cycle. The Ca/P molar ratio of HAp formed at 10 reaction cycles was very close to the theoretical value of HAp, 1.67. CHO-K1 cell adhesion, proliferation and maximum cell density on HAp plates were better on plates formed at two or five reaction cycles using 200 mM CaCl2 and 120 mM Na2HPO 4 solutions than on plates formed under other conditions. Furthermore, the adhesion ratio of CHO-K1 cells on HAp plates formed at 10 reaction cycles was about 60% of those at two or five reaction cycles.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalBiochemical Engineering Journal
Volume20
Issue number2-3
DOIs
Publication statusPublished - Aug 15 2004
Externally publishedYes

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Keywords

  • Alternate soaking process
  • Animal cell culture
  • Biomedical
  • Culture substratum
  • Hydroxyapatite
  • Optimization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Chemical Engineering(all)

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