Abstract
Hydroxyapatite (HAp), the major inorganic component of hard tissues in vivo, was formed in/on a PVA gel. The HAp formation ratio depended on the reaction cycle number, but was independent of the alternate soaking period per cycle. The Ca/P molar ratio of HAp formed at 10 reaction cycles was very close to the theoretical value of HAp, 1.67. CHO-K1 cell adhesion, proliferation and maximum cell density on HAp plates were better on plates formed at two or five reaction cycles using 200 mM CaCl2 and 120 mM Na2HPO 4 solutions than on plates formed under other conditions. Furthermore, the adhesion ratio of CHO-K1 cells on HAp plates formed at 10 reaction cycles was about 60% of those at two or five reaction cycles.
Original language | English |
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Pages (from-to) | 155-161 |
Number of pages | 7 |
Journal | Biochemical Engineering Journal |
Volume | 20 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - Aug 15 2004 |
Externally published | Yes |
Keywords
- Alternate soaking process
- Animal cell culture
- Biomedical
- Culture substratum
- Hydroxyapatite
- Optimization
ASJC Scopus subject areas
- Biotechnology
- Environmental Engineering
- Bioengineering
- Biomedical Engineering