Abstract
Hydroxyapatite (HAp), the major inorganic component of hard tissues in vivo, was formed in/on a PVA gel. The HAp formation ratio depended on the reaction cycle number, but was independent of the alternate soaking period per cycle. The Ca/P molar ratio of HAp formed at 10 reaction cycles was very close to the theoretical value of HAp, 1.67. CHO-K1 cell adhesion, proliferation and maximum cell density on HAp plates were better on plates formed at two or five reaction cycles using 200 mM CaCl2 and 120 mM Na2HPO 4 solutions than on plates formed under other conditions. Furthermore, the adhesion ratio of CHO-K1 cells on HAp plates formed at 10 reaction cycles was about 60% of those at two or five reaction cycles.
| Original language | English |
|---|---|
| Pages (from-to) | 155-161 |
| Number of pages | 7 |
| Journal | Biochemical Engineering Journal |
| Volume | 20 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Aug 15 2004 |
| Externally published | Yes |
Fingerprint
Keywords
- Alternate soaking process
- Animal cell culture
- Biomedical
- Culture substratum
- Hydroxyapatite
- Optimization
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Chemical Engineering(all)
Cite this
Hydroxyapatite for use as an animal cell culture substratum obtained by an alternate soaking process. / Ijima, Hiroyuki; Ohchi, Tomokazu; Ono, Tsutomu; Kawakami, Koei.
In: Biochemical Engineering Journal, Vol. 20, No. 2-3, 15.08.2004, p. 155-161.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hydroxyapatite for use as an animal cell culture substratum obtained by an alternate soaking process
AU - Ijima, Hiroyuki
AU - Ohchi, Tomokazu
AU - Ono, Tsutomu
AU - Kawakami, Koei
PY - 2004/8/15
Y1 - 2004/8/15
N2 - Hydroxyapatite (HAp), the major inorganic component of hard tissues in vivo, was formed in/on a PVA gel. The HAp formation ratio depended on the reaction cycle number, but was independent of the alternate soaking period per cycle. The Ca/P molar ratio of HAp formed at 10 reaction cycles was very close to the theoretical value of HAp, 1.67. CHO-K1 cell adhesion, proliferation and maximum cell density on HAp plates were better on plates formed at two or five reaction cycles using 200 mM CaCl2 and 120 mM Na2HPO 4 solutions than on plates formed under other conditions. Furthermore, the adhesion ratio of CHO-K1 cells on HAp plates formed at 10 reaction cycles was about 60% of those at two or five reaction cycles.
AB - Hydroxyapatite (HAp), the major inorganic component of hard tissues in vivo, was formed in/on a PVA gel. The HAp formation ratio depended on the reaction cycle number, but was independent of the alternate soaking period per cycle. The Ca/P molar ratio of HAp formed at 10 reaction cycles was very close to the theoretical value of HAp, 1.67. CHO-K1 cell adhesion, proliferation and maximum cell density on HAp plates were better on plates formed at two or five reaction cycles using 200 mM CaCl2 and 120 mM Na2HPO 4 solutions than on plates formed under other conditions. Furthermore, the adhesion ratio of CHO-K1 cells on HAp plates formed at 10 reaction cycles was about 60% of those at two or five reaction cycles.
KW - Alternate soaking process
KW - Animal cell culture
KW - Biomedical
KW - Culture substratum
KW - Hydroxyapatite
KW - Optimization
UR - http://www.scopus.com/inward/record.url?scp=3042612200&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042612200&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2003.07.004
DO - 10.1016/j.bej.2003.07.004
M3 - Article
VL - 20
SP - 155
EP - 161
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
IS - 2-3
ER -