Hydroxy-carbonate apatite: Synthesis, blood compatibility and adsorption of specific pathogenic proteins

Satoshi Hayakawa; Yuko Kusudo; Shinji Takemoto; Kanji Tsuru; Akiyoshi Osaka; Seisuke Takashima

Hydroxy-carbonate apatite: Synthesis, blood compatibility and adsorption of specific pathogenic proteins

Satoshi Hayakawa, Yuko Kusudo, Shinji Takemoto, Kanji Tsuru, Akiyoshi Osaka, Seisuke Takashima

Research output: Contribution to conference › Paper › peer-review

Abstract

Hydroxy-carbonate apatite (CAp) compositions containing carbonate ions from 0 to 10.9 wt% were synthesized by a wet chemical method. Infrared analysis revealed that carbonate ions were substituted both PO₄^3- and OH^- sites. The introduction of CO₃^2- ion into the hydroxyapatite lattice led to an increase in specific surface area and surface charge density. Blood clotting properties were not affected by the contact with CAp samples. The adsorption behavior of proteins was investigated by contacting CAp with model saline containing bovine serum albumin (BSA) and β₂-microglobulin (β₂-MG). The selectivity against β₂-MG increased with increase in the amount of carbonate ions. As surface charge density increased with increase in the amount of carbonate ions, surface charge density played an important role in selective adsorption of β₂-MG, but not a sole factor.

Original language	English
Pages	111-119
Number of pages	9
Publication status	Published - Dec 1 2003
Event	Bioceramics: Materials and Applications IV, Proceedings - Nashville, TN, United States Duration: Apr 27 2003 → Apr 30 2003

Conference

Conference	Bioceramics: Materials and Applications IV, Proceedings
Country/Territory	United States
City	Nashville, TN
Period	4/27/03 → 4/30/03

ASJC Scopus subject areas

Ceramics and Composites
Materials Chemistry

Cite this

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title = "Hydroxy-carbonate apatite: Synthesis, blood compatibility and adsorption of specific pathogenic proteins",

abstract = "Hydroxy-carbonate apatite (CAp) compositions containing carbonate ions from 0 to 10.9 wt% were synthesized by a wet chemical method. Infrared analysis revealed that carbonate ions were substituted both PO43- and OH- sites. The introduction of CO32- ion into the hydroxyapatite lattice led to an increase in specific surface area and surface charge density. Blood clotting properties were not affected by the contact with CAp samples. The adsorption behavior of proteins was investigated by contacting CAp with model saline containing bovine serum albumin (BSA) and β2-microglobulin (β2-MG). The selectivity against β2-MG increased with increase in the amount of carbonate ions. As surface charge density increased with increase in the amount of carbonate ions, surface charge density played an important role in selective adsorption of β2-MG, but not a sole factor.",

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T1 - Hydroxy-carbonate apatite

T2 - Bioceramics: Materials and Applications IV, Proceedings

AU - Hayakawa, Satoshi

AU - Kusudo, Yuko

AU - Takemoto, Shinji

AU - Tsuru, Kanji

AU - Osaka, Akiyoshi

AU - Takashima, Seisuke

PY - 2003/12/1

Y1 - 2003/12/1

N2 - Hydroxy-carbonate apatite (CAp) compositions containing carbonate ions from 0 to 10.9 wt% were synthesized by a wet chemical method. Infrared analysis revealed that carbonate ions were substituted both PO43- and OH- sites. The introduction of CO32- ion into the hydroxyapatite lattice led to an increase in specific surface area and surface charge density. Blood clotting properties were not affected by the contact with CAp samples. The adsorption behavior of proteins was investigated by contacting CAp with model saline containing bovine serum albumin (BSA) and β2-microglobulin (β2-MG). The selectivity against β2-MG increased with increase in the amount of carbonate ions. As surface charge density increased with increase in the amount of carbonate ions, surface charge density played an important role in selective adsorption of β2-MG, but not a sole factor.

AB - Hydroxy-carbonate apatite (CAp) compositions containing carbonate ions from 0 to 10.9 wt% were synthesized by a wet chemical method. Infrared analysis revealed that carbonate ions were substituted both PO43- and OH- sites. The introduction of CO32- ion into the hydroxyapatite lattice led to an increase in specific surface area and surface charge density. Blood clotting properties were not affected by the contact with CAp samples. The adsorption behavior of proteins was investigated by contacting CAp with model saline containing bovine serum albumin (BSA) and β2-microglobulin (β2-MG). The selectivity against β2-MG increased with increase in the amount of carbonate ions. As surface charge density increased with increase in the amount of carbonate ions, surface charge density played an important role in selective adsorption of β2-MG, but not a sole factor.

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Hydroxy-carbonate apatite: Synthesis, blood compatibility and adsorption of specific pathogenic proteins

Abstract

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ASJC Scopus subject areas

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