Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum

Hiroyuki Ijima, Motonori Nakashima, Sachiko Furuta, Tsutomu Ono, Koei Kawakami

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A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

Original languageEnglish
Pages (from-to)531-535
Number of pages5
JournalJournal of Chemical Engineering of Japan
Issue number4
Publication statusPublished - Apr 2004
Externally publishedYes



  • CHO-K1 cells
  • Culture substratum
  • Gypsum
  • Hydrothermal treatment
  • Hydroxyapatite
  • Proliferation

ASJC Scopus subject areas

  • Chemical Engineering(all)

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