Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption

Koji Matsuura, Yuka Kuroda, Keisuke Yamashita, Hiroaki Funahashi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P<0.05) and ALHD (P<0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalJournal of Reproduction and Development
Volume57
Issue number1
DOIs
Publication statusPublished - Mar 28 2011

Keywords

  • Adsorption
  • Porcine sperm motility
  • Silicone elastomer
  • Trajectories

ASJC Scopus subject areas

  • Animal Science and Zoology

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