Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption

Koji Matsuura, Yuka Kuroda, Keisuke Yamashita, Hiroaki Funahashi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalJournal of Reproduction and Development
Volume57
Issue number1
DOIs
Publication statusPublished - 2011

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Keywords

  • Adsorption
  • Porcine sperm motility
  • Silicone elastomer
  • Trajectories

ASJC Scopus subject areas

  • Animal Science and Zoology

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