Hydrolytic Action on the Mixture of Maltose and Soluble Starch by a-Glucosidase from Mucor javanicus IFO 4570

Manabu Sugimoto; Yukio Suzuki

doi:10.1271/bbb.58.1535

Hydrolytic Action on the Mixture of Maltose and Soluble Starch by a-Glucosidase from Mucor javanicus IFO 4570

Manabu Sugimoto, Yukio Suzuki

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site.

Original language	English
Pages (from-to)	1535-1536
Number of pages	2
Journal	Bioscience, Biotechnology, and Biochemistry
Volume	58
Issue number	8
DOIs	https://doi.org/10.1271/bbb.58.1535
Publication status	Published - 1994

ASJC Scopus subject areas

Analytical Chemistry
Biotechnology
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site.",

author = "Manabu Sugimoto and Yukio Suzuki",

year = "1994",

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AB - The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site.

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Hydrolytic Action on the Mixture of Maltose and Soluble Starch by a-Glucosidase from Mucor javanicus IFO 4570

Abstract

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