Hydrolytic action on the mixture of maltose and soluble starch by α-glucosidase from mucor javanicus ifo 4570

Manabu Sugimoto, Yukio Suzuki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site.

Original languageEnglish
Pages (from-to)1535-1536
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume58
Issue number8
DOIs
Publication statusPublished - 1994

Fingerprint

Glucosidases
Mucor
Maltose
Starch
Catalytic Domain
Kinetics
Substrates
Enzymes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology

Cite this

Hydrolytic action on the mixture of maltose and soluble starch by α-glucosidase from mucor javanicus ifo 4570. / Sugimoto, Manabu; Suzuki, Yukio.

In: Bioscience, Biotechnology and Biochemistry, Vol. 58, No. 8, 1994, p. 1535-1536.

Research output: Contribution to journalArticle

@article{56d9987ac49a4c868fb49537c4c70c0d,
title = "Hydrolytic action on the mixture of maltose and soluble starch by α-glucosidase from mucor javanicus ifo 4570",
abstract = "The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site.",
author = "Manabu Sugimoto and Yukio Suzuki",
year = "1994",
doi = "10.1080/bbb.58.1535",
language = "English",
volume = "58",
pages = "1535--1536",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "8",

}

TY - JOUR

T1 - Hydrolytic action on the mixture of maltose and soluble starch by α-glucosidase from mucor javanicus ifo 4570

AU - Sugimoto, Manabu

AU - Suzuki, Yukio

PY - 1994

Y1 - 1994

N2 - The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site.

AB - The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site.

UR - http://www.scopus.com/inward/record.url?scp=0005836679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0005836679&partnerID=8YFLogxK

U2 - 10.1080/bbb.58.1535

DO - 10.1080/bbb.58.1535

M3 - Article

AN - SCOPUS:0005836679

VL - 58

SP - 1535

EP - 1536

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 8

ER -