Hydrogen-rich water attenuates experimental periodontitis in a rat model

Kenta Kasuyama, Takaaki Tomofuji, Daisuke Ekuni, Naofumi Tamaki, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Manabu Morita

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aim Reactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen-rich water on a rat model of periodontitis. Material & Methods A ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen-rich water. Results The rats with periodontitis which were treated with pure water showed a time-dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis-induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen-rich water intake inhibited an increase in serum ROS level and lowered expression of 8-hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen-activated protein kinases, were less activated in periodontal lesions from hydrogen-rich water-treated rats as compared with pure water-treated rats. Conclusion Consuming hydrogen-rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

Original languageEnglish
Pages (from-to)1085-1090
Number of pages6
JournalJournal of Clinical Periodontology
Volume38
Issue number12
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Periodontitis
Hydrogen
Water
Reactive Oxygen Species
Neutrophils
Alveolar Bone Loss
Osteoclasts
Mitogen-Activated Protein Kinases
Serum
Drinking Water
Drinking
Ligation
Oxidative Stress

Keywords

  • animal studies
  • antioxidants
  • hydrogen-rich water
  • oxidative stress
  • periodontitis

ASJC Scopus subject areas

  • Periodontics

Cite this

Hydrogen-rich water attenuates experimental periodontitis in a rat model. / Kasuyama, Kenta; Tomofuji, Takaaki; Ekuni, Daisuke; Tamaki, Naofumi; Azuma, Tetsuji; Irie, Koichiro; Endo, Yasumasa; Morita, Manabu.

In: Journal of Clinical Periodontology, Vol. 38, No. 12, 12.2011, p. 1085-1090.

Research output: Contribution to journalArticle

Kasuyama, Kenta ; Tomofuji, Takaaki ; Ekuni, Daisuke ; Tamaki, Naofumi ; Azuma, Tetsuji ; Irie, Koichiro ; Endo, Yasumasa ; Morita, Manabu. / Hydrogen-rich water attenuates experimental periodontitis in a rat model. In: Journal of Clinical Periodontology. 2011 ; Vol. 38, No. 12. pp. 1085-1090.
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