Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana

Cortwa Hooijmaijers, Ji Ye Rhee, Kyung Jin Kwak, Gap Chae Chung, Tomoaki Horie, Maki Katsuhara, Hunseung Kang

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Although aquaporins have been known to transport hydrogen peroxide (H 2O 2) across cell membranes, the H 2O 2-regulated expression patterns and the permeability of every family member of the plasma membrane intrinsic protein (PIP) toward H 2O 2 have not been determined. This study investigates the H 2O 2-regulated expression levels of all plasma membrane aquaporins of Arabidopsis thaliana (AtPIPs), and determines the permeability of every AtPIP for H 2O 2 in yeast. Hydrogen peroxide treatment of Arabidopsis down-regulated the expression of AtPIP2 subfamily in roots but not in leaves, whereas the expression of AtPIP1 subfamily was not affected by H 2O 2 treatment. The growth and survival of yeast cells that expressed AtPIP2;2, AtPIP2;4, AtPIP2;5, or AtPIP2;7 was reduced in the presence of H 2O 2, while the growth of yeast cells expressing any other AtPIP family member was not affected by H 2O 2. These results show that only certain isoforms of AtPIPs whose expression is regulated by H 2O 2 treatment are permeable for H 2O 2 in yeast cells, and suggest that the integrated regulation of aquaporin expression by H 2O 2 and the capacity of individual aquaporin to transport H 2O 2 are important for plant response to H 2O 2.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalJournal of Plant Research
Volume125
Issue number1
DOIs
Publication statusPublished - Jan 2012

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aquaporins
hydrogen peroxide
permeability
plasma membrane
Arabidopsis thaliana
yeasts
cells
membrane proteins
blood proteins
cell membranes
plant response
Arabidopsis
leaves

Keywords

  • Aquaporin
  • Arabidopsis
  • Hydrogen peroxide
  • Transporter
  • Water channel

ASJC Scopus subject areas

  • Plant Science

Cite this

Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana. / Hooijmaijers, Cortwa; Rhee, Ji Ye; Kwak, Kyung Jin; Chung, Gap Chae; Horie, Tomoaki; Katsuhara, Maki; Kang, Hunseung.

In: Journal of Plant Research, Vol. 125, No. 1, 01.2012, p. 147-153.

Research output: Contribution to journalArticle

Hooijmaijers, Cortwa ; Rhee, Ji Ye ; Kwak, Kyung Jin ; Chung, Gap Chae ; Horie, Tomoaki ; Katsuhara, Maki ; Kang, Hunseung. / Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana. In: Journal of Plant Research. 2012 ; Vol. 125, No. 1. pp. 147-153.
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