Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana

Cortwa Hooijmaijers, Ji Ye Rhee, Kyung Jin Kwak, Gap Chae Chung, Tomoaki Horie, Maki Katsuhara, Hunseung Kang

    Research output: Contribution to journalArticlepeer-review

    78 Citations (Scopus)

    Abstract

    Although aquaporins have been known to transport hydrogen peroxide (H 2O 2) across cell membranes, the H 2O 2-regulated expression patterns and the permeability of every family member of the plasma membrane intrinsic protein (PIP) toward H 2O 2 have not been determined. This study investigates the H 2O 2-regulated expression levels of all plasma membrane aquaporins of Arabidopsis thaliana (AtPIPs), and determines the permeability of every AtPIP for H 2O 2 in yeast. Hydrogen peroxide treatment of Arabidopsis down-regulated the expression of AtPIP2 subfamily in roots but not in leaves, whereas the expression of AtPIP1 subfamily was not affected by H 2O 2 treatment. The growth and survival of yeast cells that expressed AtPIP2;2, AtPIP2;4, AtPIP2;5, or AtPIP2;7 was reduced in the presence of H 2O 2, while the growth of yeast cells expressing any other AtPIP family member was not affected by H 2O 2. These results show that only certain isoforms of AtPIPs whose expression is regulated by H 2O 2 treatment are permeable for H 2O 2 in yeast cells, and suggest that the integrated regulation of aquaporin expression by H 2O 2 and the capacity of individual aquaporin to transport H 2O 2 are important for plant response to H 2O 2.

    Original languageEnglish
    Pages (from-to)147-153
    Number of pages7
    JournalJournal of Plant Research
    Volume125
    Issue number1
    DOIs
    Publication statusPublished - Jan 2012

    Keywords

    • Aquaporin
    • Arabidopsis
    • Hydrogen peroxide
    • Transporter
    • Water channel

    ASJC Scopus subject areas

    • Plant Science

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