Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation

Keisuke Kohama, Hayato Yamashita, Michiko Aoyama-Ishikawa, Toru Takahashi, Timothy R. Billiar, Takeshi Nishimura, Joji Kotani, Atsunori Nakao

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Introduction Hemorrhagic shock followed by fluid resuscitation (HS/R) triggers an inflammatory response and causes pulmonary inflammation that can lead to acute lung injury (ALI). Hydrogen, a therapeutic gas, has potent cytoprotective, antiinflammatory, and antioxidant effects. This study examined the effects of inhaled hydrogen on ALI caused by HS/R. Methods Rats were subjected to hemorrhagic shock by withdrawing blood to lower blood pressure followed by resuscitation with shed blood and saline to restore blood pressure. After HS/R, the rats were maintained in a control gas of similar composition to room air or exposed to 1.3% hydrogen. Results HS/R induced ALI, as demonstrated by significantly impaired gas exchange, congestion, edema, cellular infiltration, and hemorrhage in the lungs. Hydrogen inhalation mitigated lung injury after HS/R, as indicated by significantly improved gas exchange and reduced cellular infiltration and hemorrhage. Hydrogen inhalation did not affect hemodynamic status during HS/R. Exposure to 1.3% hydrogen significantly attenuated the upregulation of the messenger RNAs for several proinflammatory mediators induced by HS/R. Lipid peroxidation was reduced significantly in the presence of hydrogen, indicating antioxidant effects. Conclusion Hydrogen, administered through inhalation, may exert potent therapeutic effects against ALI induced by HS/R and attenuate the activation of inflammatory cascades.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalSurgery (United States)
Volume158
Issue number2
DOIs
Publication statusPublished - Aug 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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    Kohama, K., Yamashita, H., Aoyama-Ishikawa, M., Takahashi, T., Billiar, T. R., Nishimura, T., Kotani, J., & Nakao, A. (2015). Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation. Surgery (United States), 158(2), 399-407. https://doi.org/10.1016/j.surg.2015.03.038