Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation

Keisuke Kohama, Hayato Yamashita, Michiko Aoyama-Ishikawa, Toru Takahashi, Timothy R. Billiar, Takeshi Nishimura, Joji Kotani, Atsunori Nakao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Introduction Hemorrhagic shock followed by fluid resuscitation (HS/R) triggers an inflammatory response and causes pulmonary inflammation that can lead to acute lung injury (ALI). Hydrogen, a therapeutic gas, has potent cytoprotective, antiinflammatory, and antioxidant effects. This study examined the effects of inhaled hydrogen on ALI caused by HS/R. Methods Rats were subjected to hemorrhagic shock by withdrawing blood to lower blood pressure followed by resuscitation with shed blood and saline to restore blood pressure. After HS/R, the rats were maintained in a control gas of similar composition to room air or exposed to 1.3% hydrogen. Results HS/R induced ALI, as demonstrated by significantly impaired gas exchange, congestion, edema, cellular infiltration, and hemorrhage in the lungs. Hydrogen inhalation mitigated lung injury after HS/R, as indicated by significantly improved gas exchange and reduced cellular infiltration and hemorrhage. Hydrogen inhalation did not affect hemodynamic status during HS/R. Exposure to 1.3% hydrogen significantly attenuated the upregulation of the messenger RNAs for several proinflammatory mediators induced by HS/R. Lipid peroxidation was reduced significantly in the presence of hydrogen, indicating antioxidant effects. Conclusion Hydrogen, administered through inhalation, may exert potent therapeutic effects against ALI induced by HS/R and attenuate the activation of inflammatory cascades.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalSurgery
Volume158
Issue number2
DOIs
Publication statusPublished - Aug 1 2015
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Acute Lung Injury
Resuscitation
Inhalation
Hydrogen
Gases
Antioxidants
Hemorrhage
Blood Pressure
Lung Injury
Therapeutic Uses
Lipid Peroxidation
Edema
Pneumonia
Anti-Inflammatory Agents
Up-Regulation
Hemodynamics
Air
Lung
Messenger RNA

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Kohama, K., Yamashita, H., Aoyama-Ishikawa, M., Takahashi, T., Billiar, T. R., Nishimura, T., ... Nakao, A. (2015). Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation. Surgery, 158(2), 399-407. https://doi.org/10.1016/j.surg.2015.03.038

Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation. / Kohama, Keisuke; Yamashita, Hayato; Aoyama-Ishikawa, Michiko; Takahashi, Toru; Billiar, Timothy R.; Nishimura, Takeshi; Kotani, Joji; Nakao, Atsunori.

In: Surgery, Vol. 158, No. 2, 01.08.2015, p. 399-407.

Research output: Contribution to journalArticle

Kohama, K, Yamashita, H, Aoyama-Ishikawa, M, Takahashi, T, Billiar, TR, Nishimura, T, Kotani, J & Nakao, A 2015, 'Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation', Surgery, vol. 158, no. 2, pp. 399-407. https://doi.org/10.1016/j.surg.2015.03.038
Kohama K, Yamashita H, Aoyama-Ishikawa M, Takahashi T, Billiar TR, Nishimura T et al. Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation. Surgery. 2015 Aug 1;158(2):399-407. https://doi.org/10.1016/j.surg.2015.03.038
Kohama, Keisuke ; Yamashita, Hayato ; Aoyama-Ishikawa, Michiko ; Takahashi, Toru ; Billiar, Timothy R. ; Nishimura, Takeshi ; Kotani, Joji ; Nakao, Atsunori. / Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation. In: Surgery. 2015 ; Vol. 158, No. 2. pp. 399-407.
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