Hydrogel-based microenvironment for modulating gland tissue morphogenesis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Biological tissue fabrication in vitro is garnering attention these days for its potential usage as a new therapeutic tool and for understanding the mechanisms underlying tissue development. At this stage, Researchers have already developed several methods to fabricate three-dimensional (3D) cell constructs with desired size and morphology. Now, we seek to determine how we can transform this simple 3D cell construct into a functional biological tissue in vitro. Applying external stimuli to the cell construct may be one way to achieve this goal. In this context, we modified biocompatible hydrogel materials to reproduce the biomimetic physical and chemical environment. Hydrogels with different mechanical stiffness were prepared to physically mimic environments, and RGD-modified hydrogels were prepared to chemically mimic environments. To understand the effect of these synthesized environments on tissue generation and growth, the organotypic culture of mouse salivary gland tissue was studied using the environments. The potential use of the biomimetic environments synthesized by biocompatible hydrogel material for modulating tissue growth is discussed in this chapter.

Original languageEnglish
Title of host publicationHyper Bio Assembler for 3D Cellular Systems
PublisherSpringer Japan
Pages251-259
Number of pages9
ISBN (Print)9784431552970, 9784431552963
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Hydrogel
Morphogenesis
Hydrogels
Tissue
Biomimetics
Biocompatible Materials
Growth
Salivary Glands
Stiffness
Research Personnel
Fabrication

Keywords

  • Biomimetic environment
  • Cell manipulation
  • Hydrogel
  • Submandibular gland
  • Tissue manipulation

ASJC Scopus subject areas

  • Engineering(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Matsumoto, T. (2015). Hydrogel-based microenvironment for modulating gland tissue morphogenesis. In Hyper Bio Assembler for 3D Cellular Systems (pp. 251-259). Springer Japan. https://doi.org/10.1007/978-4-431-55297-0_15

Hydrogel-based microenvironment for modulating gland tissue morphogenesis. / Matsumoto, Takuya.

Hyper Bio Assembler for 3D Cellular Systems. Springer Japan, 2015. p. 251-259.

Research output: Chapter in Book/Report/Conference proceedingChapter

Matsumoto, T 2015, Hydrogel-based microenvironment for modulating gland tissue morphogenesis. in Hyper Bio Assembler for 3D Cellular Systems. Springer Japan, pp. 251-259. https://doi.org/10.1007/978-4-431-55297-0_15
Matsumoto, Takuya. / Hydrogel-based microenvironment for modulating gland tissue morphogenesis. Hyper Bio Assembler for 3D Cellular Systems. Springer Japan, 2015. pp. 251-259
@inbook{04233c8ff797449ebe685c1c705de886,
title = "Hydrogel-based microenvironment for modulating gland tissue morphogenesis",
abstract = "Biological tissue fabrication in vitro is garnering attention these days for its potential usage as a new therapeutic tool and for understanding the mechanisms underlying tissue development. At this stage, Researchers have already developed several methods to fabricate three-dimensional (3D) cell constructs with desired size and morphology. Now, we seek to determine how we can transform this simple 3D cell construct into a functional biological tissue in vitro. Applying external stimuli to the cell construct may be one way to achieve this goal. In this context, we modified biocompatible hydrogel materials to reproduce the biomimetic physical and chemical environment. Hydrogels with different mechanical stiffness were prepared to physically mimic environments, and RGD-modified hydrogels were prepared to chemically mimic environments. To understand the effect of these synthesized environments on tissue generation and growth, the organotypic culture of mouse salivary gland tissue was studied using the environments. The potential use of the biomimetic environments synthesized by biocompatible hydrogel material for modulating tissue growth is discussed in this chapter.",
keywords = "Biomimetic environment, Cell manipulation, Hydrogel, Submandibular gland, Tissue manipulation",
author = "Takuya Matsumoto",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/978-4-431-55297-0_15",
language = "English",
isbn = "9784431552970",
pages = "251--259",
booktitle = "Hyper Bio Assembler for 3D Cellular Systems",
publisher = "Springer Japan",

}

TY - CHAP

T1 - Hydrogel-based microenvironment for modulating gland tissue morphogenesis

AU - Matsumoto, Takuya

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Biological tissue fabrication in vitro is garnering attention these days for its potential usage as a new therapeutic tool and for understanding the mechanisms underlying tissue development. At this stage, Researchers have already developed several methods to fabricate three-dimensional (3D) cell constructs with desired size and morphology. Now, we seek to determine how we can transform this simple 3D cell construct into a functional biological tissue in vitro. Applying external stimuli to the cell construct may be one way to achieve this goal. In this context, we modified biocompatible hydrogel materials to reproduce the biomimetic physical and chemical environment. Hydrogels with different mechanical stiffness were prepared to physically mimic environments, and RGD-modified hydrogels were prepared to chemically mimic environments. To understand the effect of these synthesized environments on tissue generation and growth, the organotypic culture of mouse salivary gland tissue was studied using the environments. The potential use of the biomimetic environments synthesized by biocompatible hydrogel material for modulating tissue growth is discussed in this chapter.

AB - Biological tissue fabrication in vitro is garnering attention these days for its potential usage as a new therapeutic tool and for understanding the mechanisms underlying tissue development. At this stage, Researchers have already developed several methods to fabricate three-dimensional (3D) cell constructs with desired size and morphology. Now, we seek to determine how we can transform this simple 3D cell construct into a functional biological tissue in vitro. Applying external stimuli to the cell construct may be one way to achieve this goal. In this context, we modified biocompatible hydrogel materials to reproduce the biomimetic physical and chemical environment. Hydrogels with different mechanical stiffness were prepared to physically mimic environments, and RGD-modified hydrogels were prepared to chemically mimic environments. To understand the effect of these synthesized environments on tissue generation and growth, the organotypic culture of mouse salivary gland tissue was studied using the environments. The potential use of the biomimetic environments synthesized by biocompatible hydrogel material for modulating tissue growth is discussed in this chapter.

KW - Biomimetic environment

KW - Cell manipulation

KW - Hydrogel

KW - Submandibular gland

KW - Tissue manipulation

UR - http://www.scopus.com/inward/record.url?scp=84943372340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943372340&partnerID=8YFLogxK

U2 - 10.1007/978-4-431-55297-0_15

DO - 10.1007/978-4-431-55297-0_15

M3 - Chapter

AN - SCOPUS:84943372340

SN - 9784431552970

SN - 9784431552963

SP - 251

EP - 259

BT - Hyper Bio Assembler for 3D Cellular Systems

PB - Springer Japan

ER -