Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis

Hiroyuki Miyajima, Takuya Matsumoto, Takayoshi Sakai, Satoshi Yamaguchi, Sang Hyun An, Makoto Abe, Satoshi Wakisaka, Kuen Yong Lee, Hiroshi Egusa, Satoshi Imazato

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The mechanical properties of the cellular microenvironment dramatically alter during tissue development and growth. Growing evidence suggests that physical microenvironments and mechanical stresses direct cell fate in developing tissue. However, how these physical cues affect the tissue morphogenesis remains a major unknown. We explain here that the physical properties of the cell and tissue microenvironment, biomimetically reproduced by using hydrogel, guide the tissue morphogenesis in the developmental submandibular gland (SMG). In particular, the softer gel enhances the bud expansion and cleft formation of SMG, whereas the stiffer gel attenuates them. These morphological changes in SMG tissue are led by soluble factors (FGF7/10) induction regulated by cell traction force derived from the tissue deformation. Our findings suggest that cells sense the mechanics of their surrounding environment and alter their properties for self-organization and the following tissue morphogenesis. Also, physically designed hydrogel material is a valuable tool for producing the biomimetic microenvironment to explore how physical cues affect tissue morphogenesis and to modulate tissue morphogenesis for . in vitro tissue synthesis.

Original languageEnglish
Pages (from-to)6754-6763
Number of pages10
JournalBiomaterials
Volume32
Issue number28
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Fingerprint

Biomimetics
Hydrogel
Morphogenesis
Hydrogels
Modulation
Tissue
Submandibular Gland
Cellular Microenvironment
Cues
In Vitro Techniques
Gels
Mechanical Stress
Traction
Mechanics
Growth and Development
Physical properties

Keywords

  • Alginate
  • Biomimetic material
  • Mechanical properties
  • Morphogenesis
  • Salivary gland

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis. / Miyajima, Hiroyuki; Matsumoto, Takuya; Sakai, Takayoshi; Yamaguchi, Satoshi; An, Sang Hyun; Abe, Makoto; Wakisaka, Satoshi; Lee, Kuen Yong; Egusa, Hiroshi; Imazato, Satoshi.

In: Biomaterials, Vol. 32, No. 28, 10.2011, p. 6754-6763.

Research output: Contribution to journalArticle

Miyajima, H, Matsumoto, T, Sakai, T, Yamaguchi, S, An, SH, Abe, M, Wakisaka, S, Lee, KY, Egusa, H & Imazato, S 2011, 'Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis', Biomaterials, vol. 32, no. 28, pp. 6754-6763. https://doi.org/10.1016/j.biomaterials.2011.05.072
Miyajima, Hiroyuki ; Matsumoto, Takuya ; Sakai, Takayoshi ; Yamaguchi, Satoshi ; An, Sang Hyun ; Abe, Makoto ; Wakisaka, Satoshi ; Lee, Kuen Yong ; Egusa, Hiroshi ; Imazato, Satoshi. / Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis. In: Biomaterials. 2011 ; Vol. 32, No. 28. pp. 6754-6763.
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