Hydrocortisone stimulation of proliferation and glycosaminoglycan synthesis in rabbit craniofacial chondrocytes in vitro

Masaharu Takigawa, T. Takano, K. Nakagawa, M. Sakuda, F. Suzuki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Hydrocortisone stimulated glycosaminoglycan (GAG) synthesis, a cartilage phenotype, in chondrocytes from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC) and spheno-occipital synchondrosis (SOS). These stimulations were dose- and time-dependent, being maximal 27 h after addition of 10-7M hydrocortisone. The maximal induced increase of GAG synthesis was about 100%, 50% and 20% that of non-stimulated MCC, SOS and NSC chondrocytes, respectively. When stained with toluidine blue, all three types of cortisone-treated chondrocytes showed stronger metachromasia than non-treated controls. DNA synthesis was also increased by hydrocortisone, reaching a maximum 20 h after the addition; stimulation was also dose-dependent and maximal at a concentration of 10-6M. The maximal increase in DNA synthesis was 200% in NSC chondrocytes, 90% in SOS chondrocytes, and slight in MCC chondrocytes. However, there was no stimulation of DNA synthesis in serum-free medium, in contrast to that of GAG synthesis. These observations suggest that hydrocortisone regulates craniofacial growth by controlling the differentiation of these chondrocytes directly and their proliferation indirectly, and that the difference in their responses to hydrocortisone may reflect different responses in vivo.

Original languageEnglish
Pages (from-to)893-899
Number of pages7
JournalArchives of Oral Biology
Volume33
Issue number12
DOIs
Publication statusPublished - 1988
Externally publishedYes

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Chondrocytes
Glycosaminoglycans
Hydrocortisone
Rabbits
Nasal Cartilages
Cartilage
DNA
Tolonium Chloride
Cortisone
Serum-Free Culture Media
In Vitro Techniques
Phenotype
Growth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Hydrocortisone stimulation of proliferation and glycosaminoglycan synthesis in rabbit craniofacial chondrocytes in vitro. / Takigawa, Masaharu; Takano, T.; Nakagawa, K.; Sakuda, M.; Suzuki, F.

In: Archives of Oral Biology, Vol. 33, No. 12, 1988, p. 893-899.

Research output: Contribution to journalArticle

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